All SitesHuman: Thr433; Mouse: Thr426
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human E2F1 around the phosphorylation site of threonine 433 (D-L-TP-P-L).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityE2F1 (Phospho-Thr433) antibody detects endogenous levels of E2F1 only when phosphorylated at threonine 433.
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Ludger Hauck, Mol. Cell. Biol., Apr 2002; 22: 2147 - 2158.
Kang Liu, Mol. Cell. Biol., May 2003; 23: 3287 - 3304.
J K Hsieh, Genes & Dev., Jul 1997; 11: 1840 - 1852.