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Primary Antibodies

E2F1 (Phospho-Thr433) Antibody

E11-0423ACite ID: EN0017036DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 ELISA: 1:5000
Q01094
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All SitesHuman: Thr433; Mouse: Thr426

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human E2F1 around the phosphorylation site of threonine 433 (D-L-TP-P-L).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityE2F1 (Phospho-Thr433) antibody detects endogenous levels of E2F1 only when phosphorylated at threonine 433.
ReferencesJing W. Zhu, Mol. Cell. Biol., Dec 2001; 21: 8547 - 8564.
Ludger Hauck, Mol. Cell. Biol., Apr 2002; 22: 2147 - 2158.
Kang Liu, Mol. Cell. Biol., May 2003; 23: 3287 - 3304.
J K Hsieh, Genes & Dev., Jul 1997; 11: 1840 - 1852.
Price225$
Western blot analysis of extracts from HeLa cells, treated with Etoposide (25μM, 24hours), using E2F1 (Phospho- Thr433) Antibody
Western blot analysis of extracts from HeLa cells, treated with Etoposide (25μM, 24hours), using E2F1 (Phospho- Thr433) Antibody

Conjugated Antibody

This product is also available conjugated. Format: E11-0423A-Conjugate. Price: $485 / Size: 100μl.

E11-0423A-FITC$485 / 100μlE11-0423A-Biotin$485 / 100μlE11-0423A-HRP$485 / 100μlE11-0423A-AP$485 / 100μlE11-0423A-AF350$485 / 100μlE11-0423A-AF405M$485 / 100μlE11-0423A-AF488$485 / 100μlE11-0423A-AF514$485 / 100μlE11-0423A-AF532$485 / 100μlE11-0423A-AF555$485 / 100μlE11-0423A-AF568$485 / 100μlE11-0423A-AF405S$485 / 100μlE11-0423A-AF405L$485 / 100μlE11-0423A-AF546$485 / 100μlE11-0423A-AF594$485 / 100μlE11-0423A-AF610$485 / 100μlE11-0423A-AF635$485 / 100μlE11-0423A-AF647$485 / 100μlE11-0423A-AF680$485 / 100μlE11-0423A-AF700$485 / 100μlE11-0423A-AF750$485 / 100μlE11-0423A-AF790$485 / 100μlE11-0423A-APC$485 / 100μlE11-0423A-PE$485 / 100μlE11-0423A-Cy3$485 / 100μlE11-0423A-Cy5$485 / 100μlE11-0423A-Cy5.5$485 / 100μlE11-0423A-Cy7$485 / 100μlE11-0423A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

E2F1 (Phospho-Thr433) Antibody | EnoGene Biotech Co. Ltd