All Names
AlternameCHEK2, CHK2, Cds1, Chk2, RAD53, kinase Chk2
All SitesHuman: Thr387; Mouse: Thr391; Rat: Thr390
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 387 (C-G-TP-P-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityChk2 (Phospho-Thr387) antibody detects endogenous levels of Chk2 only when phosphorylated at threonine 387.
ReferencesAtsushi Hirao, Mol. Cell. Biol., Sep 2002; 22: 6521 - 6532.
Julie K. Schwarz, Mol. Cancer Res., Jun 2003; 1: 598.
Shutong Yang, J. Biol. Chem., Sep 2006; 281: 26645 - 26654.
Xingzhi Xu, Mol. Cell. Biol., Jun 2002; 22: 4419 - 4432.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells