Other Names Activating transcription factor 1; ATF1; Cyclic-AMP-dependent transcription factor ATF-1; TCR-ATF1; TREB36 protein
All Sites Human: Ser63; Mouse: Ser63
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ATF1 around the phosphorylation site of serine 63 (R-P-SP-Y-R).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity ATF1 (Phospho-Ser63) antibody detects endogenous levels of ATF1 only when phosphorylated at serine 63.
ReferencesYoshimura T., EMBO J. 9:2537-2542(1990).
Rehfuss R.P., J. Biol. Chem. 266:18431-18434(1991).
Hai T., Genes Dev. 3:2083-2090(1989).