Other Names AKT, AKT1 kinase, C-AKT, PKB, PKB-alpha, Protein kinase B, RAC, RAC-PK-alpha, RAC-alpha serine/threonine kinase, kinase Akt1
All Sites Human: Ser124; Mouse: Ser124; Rat: Ser124
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 124 (S-G-SP-P-S).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Akt (Phospho-Ser124) antibody detects endogenous levels of Akt only when phosphorylated at serine 124.
ReferencesPayaningal R. Somanath, J. Biol. Chem., Jun 2007; 10.1074.
Xiaoming Ju, PNAS, May 2007; 104: 7438 - 7443.
Brian DeBosch, Circulation, May 2 006; 113: 2097 - 2104.