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Primary Antibodies

4E-BP1 (Phospho-Ser64) Antibody

E11-0401ACite ID: EN0016994DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:1000
Q13541
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

Altername4EBP1, EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Insulin-stimulated EIF-4E binding protein PHAS-I, P/OKCL.6, PHAS-1, PHAS-I
All SitesHuman: Ser64; Mouse: Ser63; Rat: Ser63

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of serine 64 (R-N-SP-P-V).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity4E-BP1 (Phospho-Ser64) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at serine 64.
ReferencesAnne-Claude Gingras, Genes & Dev., Jun 1999; 13: 1422.
Guangming Liu, J. Biol. Chem., Mar 2002; 277: 8810 - 8816.
Ya-Ping Jiang, J. Biol. Chem., Mar 2001; 276: 10943 - 10951.
Anne-Claude Gingras, Genes & Dev., Feb 1998; 12: 502.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho- Ser64) antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho- Ser64) antibody.
Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Phospho- Ser64) antibody.
Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Phospho- Ser64) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0401A-Conjugate. Price: $485 / Size: 100μl.

E11-0401A-FITC$485 / 100μlE11-0401A-Biotin$485 / 100μlE11-0401A-HRP$485 / 100μlE11-0401A-AP$485 / 100μlE11-0401A-AF350$485 / 100μlE11-0401A-AF405M$485 / 100μlE11-0401A-AF488$485 / 100μlE11-0401A-AF514$485 / 100μlE11-0401A-AF532$485 / 100μlE11-0401A-AF555$485 / 100μlE11-0401A-AF568$485 / 100μlE11-0401A-AF405S$485 / 100μlE11-0401A-AF405L$485 / 100μlE11-0401A-AF546$485 / 100μlE11-0401A-AF594$485 / 100μlE11-0401A-AF610$485 / 100μlE11-0401A-AF635$485 / 100μlE11-0401A-AF647$485 / 100μlE11-0401A-AF680$485 / 100μlE11-0401A-AF700$485 / 100μlE11-0401A-AF750$485 / 100μlE11-0401A-AF790$485 / 100μlE11-0401A-APC$485 / 100μlE11-0401A-PE$485 / 100μlE11-0401A-Cy3$485 / 100μlE11-0401A-Cy5$485 / 100μlE11-0401A-Cy5.5$485 / 100μlE11-0401A-Cy7$485 / 100μlE11-0401A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.