All Names
AlternameWNK lysine deficient protein kinase 1, KDP, PRKWNK1 PROTEIN KINASE LYSINE-DEFICIENT 1
All SitesHuman: Thr60; Mouse: Thr58; Rat: Thr58
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human WNK1 around the phosphorylation site of threonine 58 (R-H-TP-M-D).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityWNK1 (Phospho-Thr58) antibody detects endogenous levels of WNK1 only when phosphorylated at threonine 58.
ReferencesDiana Pacheco-Alvarez, J. Biol. Chem., Sep 2006; 281: 28755 - 28763.
Henrik Dimke, Am J Physiol Renal Physiol, Feb 2007; 292: F723 - F735.