All Names
AlternameSpleen tyrosine kinase, PROTEIN-TYROSINE KINASE SYK
All SitesHuman: Tyr525; Mouse: Tyr519; Rat: Tyr519
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human SYK around the phosphorylation site of tyrosine 525 (E-N-YP-Y-K).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivitySYK (Phospho-Tyr525) antibody detects endogenous levels of SYK only when phosphorylated at tyrosine 525.
ReferencesLudivine Leseux, Blood, Dec 2006; 108: 4156 - 4162.
Carol A. Dangelmaier, Blood, May 2005; 105: 3918 - 3924.
Carol A. Dangelmaier, Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 627.
Clément Couture, J. Biol. Chem., Sep 1996; 271: 24294.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells