Other Names Caspase-like protein ESPL1, EC 3.4.22.49, ESP1, ESPL1, Extra spindle poles-like 1 protein, KIAA0165, Separin
All Sites Human: Ser801; Mouse: Ser1189
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human SEPARASE around the phosphorylation site of serine 801 (N-S-SP-P-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity SEPARASE (Phospho-Ser801) antibody detects endogenous levels of SEPARASE only when phosphorylated at serine 801.
ReferencesWaizenegger I., Curr. Biol. 12:1368-1378(2002).
Nagase T., DNA Res. 3:17-24(1996).
Zou H., Science 285:418-422(1999).