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Primary Antibodies

POLR2A (Phospho-Ser1619) Antibody

E11-0082ACite ID: EN0016573DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1: 500~1:1000 IHC: 1:50~1:100
IF: 1:100~1:500 ELISA: 1:10000
P24928
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameRPO2; RPOL2,POLR2 ALPHA-AMANITIN RESISTANCE,POLYMERASE II, RNA, SUBUNIT A,RNA POLYMERASE II, 220-KD SUBUNIT,RNA POLYMERASE II, LARGE SUBUNIT,RPB1, S. CEREVISIAE, HOMOLOG OF polymerase (RNA) II (DNA directed) polypeptide A
All SitesHuman: Ser1619; Mouse: Ser1619; Rat: Ser1598

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human POLR2A around the phosphorylation site of serine 1619 (P-T-SP-P-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityPOLR2A (phospho-Ser1619) antibody detects endogenous levels of POLR2A only when phosphorylated at serine 1619.
ReferencesTracy Jo Pasieka, J. Virol., Aug 2006; 80: 7600 - 7612.
Olaf R. Mook, Mol. Cancer Ther., Mar 2007; 6: 833 - 843.
Colleen A. McMullen, J. Neurosci., Jan 2004; 24: 161.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A (phospho- Ser1619) antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A (phospho- Ser1619) antibody.
Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30mins), using POLR2A (phospho- Ser1619) antibody.
Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30mins), using POLR2A (phospho- Ser1619) antibody.
Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using POLR2A (phospho- Ser1619) antibody.
Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using POLR2A (phospho- Ser1619) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0082A-Conjugate. Price: $485 / Size: 100μl.

E11-0082A-FITC$485 / 100μlE11-0082A-Biotin$485 / 100μlE11-0082A-HRP$485 / 100μlE11-0082A-AP$485 / 100μlE11-0082A-AF350$485 / 100μlE11-0082A-AF405M$485 / 100μlE11-0082A-AF488$485 / 100μlE11-0082A-AF514$485 / 100μlE11-0082A-AF532$485 / 100μlE11-0082A-AF555$485 / 100μlE11-0082A-AF568$485 / 100μlE11-0082A-AF405S$485 / 100μlE11-0082A-AF405L$485 / 100μlE11-0082A-AF546$485 / 100μlE11-0082A-AF594$485 / 100μlE11-0082A-AF610$485 / 100μlE11-0082A-AF635$485 / 100μlE11-0082A-AF647$485 / 100μlE11-0082A-AF680$485 / 100μlE11-0082A-AF700$485 / 100μlE11-0082A-AF750$485 / 100μlE11-0082A-AF790$485 / 100μlE11-0082A-APC$485 / 100μlE11-0082A-PE$485 / 100μlE11-0082A-Cy3$485 / 100μlE11-0082A-Cy5$485 / 100μlE11-0082A-Cy5.5$485 / 100μlE11-0082A-Cy7$485 / 100μlE11-0082A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.