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Primary Antibodies

eEF2 (Phospho-Thr56) Antibody

E11-0070ACite ID: EN0016548DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1000 IHC: 1:50~100
IF: 1:100~1:500 ELISA: 1:10000
P13639
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameEEF2, EF-2, EF2, Elongation factor 2
All SitesHuman: Thr56; Mouse: Thr56; Rat: Thr56

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human eEF2 around the phosphorylation site of threonine 56 (R-F-TP-D-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityeEF2 (Phospho-Thr56) antibody detects endogenous levels of eEF2 only when phosphorylated at threonine 56.
ReferencesDavid M. Thomson, FASEB J, Mar 2006; 20: A819.
Scott E. Gordon, FASEB J, Mar 2006; 20: A819.
Xu Li, FEBS J., Aug 2005; 272: 4211 - 4220.
Adam J. Rose, J. Physiol., Nov 2005; 569: 223 - 228.
Price225$
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eEF2 (Phospho- Thr56) antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eEF2 (Phospho- Thr56) antibody.
Western blot analysis of extracts from NIH/3T3 cells treated with serum (10%, 30mins), using eEF2 (Phospho- Thr56) antibody.
Western blot analysis of extracts from NIH/3T3 cells treated with serum (10%, 30mins), using eEF2 (Phospho- Thr56) antibody.
Immunofluorescence analysis of HuvEc cells, using eEF2 (Phospho-Thr56) antibody (#A0070).
Immunofluorescence analysis of HuvEc cells, using eEF2 (Phospho-Thr56) antibody (#A0070).

Conjugated Antibody

This product is also available conjugated. Format: E11-0070A-Conjugate. Price: $485 / Size: 100μl.

E11-0070A-FITC$485 / 100μlE11-0070A-Biotin$485 / 100μlE11-0070A-HRP$485 / 100μlE11-0070A-AP$485 / 100μlE11-0070A-AF350$485 / 100μlE11-0070A-AF405M$485 / 100μlE11-0070A-AF488$485 / 100μlE11-0070A-AF514$485 / 100μlE11-0070A-AF532$485 / 100μlE11-0070A-AF555$485 / 100μlE11-0070A-AF568$485 / 100μlE11-0070A-AF405S$485 / 100μlE11-0070A-AF405L$485 / 100μlE11-0070A-AF546$485 / 100μlE11-0070A-AF594$485 / 100μlE11-0070A-AF610$485 / 100μlE11-0070A-AF635$485 / 100μlE11-0070A-AF647$485 / 100μlE11-0070A-AF680$485 / 100μlE11-0070A-AF700$485 / 100μlE11-0070A-AF750$485 / 100μlE11-0070A-AF790$485 / 100μlE11-0070A-APC$485 / 100μlE11-0070A-PE$485 / 100μlE11-0070A-Cy3$485 / 100μlE11-0070A-Cy5$485 / 100μlE11-0070A-Cy5.5$485 / 100μlE11-0070A-Cy7$485 / 100μlE11-0070A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.