All Names
AlternameEEF2, EF-2, EF2, Elongation factor 2
All SitesHuman: Thr56; Mouse: Thr56; Rat: Thr56
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human eEF2 around the phosphorylation site of threonine 56 (R-F-TP-D-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityeEF2 (Phospho-Thr56) antibody detects endogenous levels of eEF2 only when phosphorylated at threonine 56.
ReferencesDavid M. Thomson, FASEB J, Mar 2006; 20: A819.
Scott E. Gordon, FASEB J, Mar 2006; 20: A819.
Xu Li, FEBS J., Aug 2005; 272: 4211 - 4220.
Adam J. Rose, J. Physiol., Nov 2005; 569: 223 - 228.