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Primary Antibodies

Cyclin B1 (Phospho-Ser126) Antibody

E11-0068ACite ID: EN0016544DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1000 IHC: 1:50~100
ELISA: 1:5000
P14635
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameCCN-2, CCNB, CCNB1, CCNB1-RS1, CYCB, CYCB1, G2/mitotic-specific cyclin B1
All SitesHuman: Ser126; Mouse: Ser123

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-SP-P-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityCyclin B1 (Phospho-Ser126) antibody detects endogenous levels of Cyclin B1 only when phosphorylated at serine 126.
ReferencesJing Yang, J. Biol. Chem., Jan 2001; 276: 3604 - 3609.
Susan Walsh, Mol. Cancer Res., Feb 2003; 1: 280.
Florian Bassermann, J. Biol. Chem., Mar 2007; 10.1074.
Jianke Li, PNAS, Jan 1997; 94: 502.
Price225$
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cyclin B1 (Phospho- Ser126) antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cyclin B1 (Phospho- Ser126) antibody.
Western blot analysis of extracts from NIH/3T3 cells, treated with EGF (200ng/ml, 15mins), using Cyclin B1 (Phospho-Ser126) antibody.
Western blot analysis of extracts from NIH/3T3 cells, treated with EGF (200ng/ml, 15mins), using Cyclin B1 (Phospho-Ser126) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0068A-Conjugate. Price: $485 / Size: 100μl.

E11-0068A-FITC$485 / 100μlE11-0068A-Biotin$485 / 100μlE11-0068A-HRP$485 / 100μlE11-0068A-AP$485 / 100μlE11-0068A-AF350$485 / 100μlE11-0068A-AF405M$485 / 100μlE11-0068A-AF488$485 / 100μlE11-0068A-AF514$485 / 100μlE11-0068A-AF532$485 / 100μlE11-0068A-AF555$485 / 100μlE11-0068A-AF568$485 / 100μlE11-0068A-AF405S$485 / 100μlE11-0068A-AF405L$485 / 100μlE11-0068A-AF546$485 / 100μlE11-0068A-AF594$485 / 100μlE11-0068A-AF610$485 / 100μlE11-0068A-AF635$485 / 100μlE11-0068A-AF647$485 / 100μlE11-0068A-AF680$485 / 100μlE11-0068A-AF700$485 / 100μlE11-0068A-AF750$485 / 100μlE11-0068A-AF790$485 / 100μlE11-0068A-APC$485 / 100μlE11-0068A-PE$485 / 100μlE11-0068A-Cy3$485 / 100μlE11-0068A-Cy5$485 / 100μlE11-0068A-Cy5.5$485 / 100μlE11-0068A-Cy7$485 / 100μlE11-0068A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.