All Names
AlternameAdapter molecule crk, CRKO, Crk-II, P38, Proto-oncogene C-crk
All SitesHuman: Tyr221; Mouse: Tyr221; Rat: Tyr221
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human CrkII around the phosphorylation site of tyrosine 221 (G-P-YP-A-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityCrkII (phospho-Tyr221) antibody detects endogenous levels of CrkII only when phosphorylated at tyrosine 221.
ReferencesRobert F. Spurney, J. Clin. Invest., May 2002; 109: 1361.
Faiyaz Ahmad, J. Immunol., Apr 1999; 162: 4864.
Feng Liu, J. Biol. Chem., Dec 1996; 271: 31290.
Jaap J. Van Hellemond, J. Biol. Chem., Mar 2000; 275: 8315.