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Primary Antibodies

CrkII (Phospho-Tyr221) Antibody

E11-0066ACite ID: EN0016540DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IF: 1:100~1:500
ELISA: 1:40000
P46108
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameAdapter molecule crk, CRKO, Crk-II, P38, Proto-oncogene C-crk
All SitesHuman: Tyr221; Mouse: Tyr221; Rat: Tyr221

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human CrkII around the phosphorylation site of tyrosine 221 (G-P-YP-A-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityCrkII (phospho-Tyr221) antibody detects endogenous levels of CrkII only when phosphorylated at tyrosine 221.
ReferencesRobert F. Spurney, J. Clin. Invest., May 2002; 109: 1361.
Faiyaz Ahmad, J. Immunol., Apr 1999; 162: 4864.
Feng Liu, J. Biol. Chem., Dec 1996; 271: 31290.
Jaap J. Van Hellemond, J. Biol. Chem., Mar 2000; 275: 8315.
Price225$
Western blot analysis of extracts from cos7 cells, CrkII (phospho-Tyr221) antibody.
Western blot analysis of extracts from cos7 cells, CrkII (phospho-Tyr221) antibody.
using Immunofluorescence analysis of HuvEc cells, using CrkII (phospho-Tyr221) antibody.
using Immunofluorescence analysis of HuvEc cells, using CrkII (phospho-Tyr221) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0066A-Conjugate. Price: $485 / Size: 100μl.

E11-0066A-FITC$485 / 100μlE11-0066A-Biotin$485 / 100μlE11-0066A-HRP$485 / 100μlE11-0066A-AP$485 / 100μlE11-0066A-AF350$485 / 100μlE11-0066A-AF405M$485 / 100μlE11-0066A-AF488$485 / 100μlE11-0066A-AF514$485 / 100μlE11-0066A-AF532$485 / 100μlE11-0066A-AF555$485 / 100μlE11-0066A-AF568$485 / 100μlE11-0066A-AF405S$485 / 100μlE11-0066A-AF405L$485 / 100μlE11-0066A-AF546$485 / 100μlE11-0066A-AF594$485 / 100μlE11-0066A-AF610$485 / 100μlE11-0066A-AF635$485 / 100μlE11-0066A-AF647$485 / 100μlE11-0066A-AF680$485 / 100μlE11-0066A-AF700$485 / 100μlE11-0066A-AF750$485 / 100μlE11-0066A-AF790$485 / 100μlE11-0066A-APC$485 / 100μlE11-0066A-PE$485 / 100μlE11-0066A-Cy3$485 / 100μlE11-0066A-Cy5$485 / 100μlE11-0066A-Cy5.5$485 / 100μlE11-0066A-Cy7$485 / 100μlE11-0066A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.