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Primary Antibodies

COT (Phospho-Thr290) Antibody

E11-0064ACite ID: EN0016536DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
IF: 1:100~1:500 ELISA: 1:40000
P41279
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameEC 1.14.16.2, TH isoform 3, TH isoform a, TH-4, TY3H, TYH, Tyrosine 3-hydroxylase, Tyrosine 5-monooxygenase, tyrosine hydroxylase
All SitesHuman: Thr290; Mouse: Thr290; Rat: Thr290

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human COT around the phosphorylation site of threonine 290 (R-G-TP-E-I).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityCOT (phospho-Thr290) antibody detects endogenous levels of COT only when phosphorylated at threonine 290.
ReferencesJahan Ara, PNAS, Jun 1998; 95: 7659.
E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107.
P. William Conrad, J. Biol. Chem., Nov 1999; 274: 33709.
Y. Katayama, J Appl Physiol, Nov 1994; 77: 2086.
Price225$
Immunohistochemical analysis of paraffin-embedded human brain tissue using COT (phospho-Thr290) antibody.
Immunohistochemical analysis of paraffin-embedded human brain tissue using COT (phospho-Thr290) antibody.
Western blot analysis of extracts from 293 cells, treated with UV (15mins), using COT (phospho-Thr290) antibody.
Western blot analysis of extracts from 293 cells, treated with UV (15mins), using COT (phospho-Thr290) antibody.
Immunofluorescence analysis of HuvEc cells, using COT (phospho-Thr290) antibody.
Immunofluorescence analysis of HuvEc cells, using COT (phospho-Thr290) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0064A-Conjugate. Price: $485 / Size: 100μl.

E11-0064A-FITC$485 / 100μlE11-0064A-Biotin$485 / 100μlE11-0064A-HRP$485 / 100μlE11-0064A-AP$485 / 100μlE11-0064A-AF350$485 / 100μlE11-0064A-AF405M$485 / 100μlE11-0064A-AF488$485 / 100μlE11-0064A-AF514$485 / 100μlE11-0064A-AF532$485 / 100μlE11-0064A-AF555$485 / 100μlE11-0064A-AF568$485 / 100μlE11-0064A-AF405S$485 / 100μlE11-0064A-AF405L$485 / 100μlE11-0064A-AF546$485 / 100μlE11-0064A-AF594$485 / 100μlE11-0064A-AF610$485 / 100μlE11-0064A-AF635$485 / 100μlE11-0064A-AF647$485 / 100μlE11-0064A-AF680$485 / 100μlE11-0064A-AF700$485 / 100μlE11-0064A-AF750$485 / 100μlE11-0064A-AF790$485 / 100μlE11-0064A-APC$485 / 100μlE11-0064A-PE$485 / 100μlE11-0064A-Cy3$485 / 100μlE11-0064A-Cy5$485 / 100μlE11-0064A-Cy5.5$485 / 100μlE11-0064A-Cy7$485 / 100μlE11-0064A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.