All Names
AlternameEC 1.14.16.2, TH isoform 3, TH isoform a, TH-4, TY3H, TYH, Tyrosine 3-hydroxylase, Tyrosine 5-monooxygenase, tyrosine hydroxylase
All SitesHuman: Thr290; Mouse: Thr290; Rat: Thr290
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human COT around the phosphorylation site of threonine 290 (R-G-TP-E-I).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityCOT (phospho-Thr290) antibody detects endogenous levels of COT only when phosphorylated at threonine 290.
ReferencesJahan Ara, PNAS, Jun 1998; 95: 7659.
E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107.
P. William Conrad, J. Biol. Chem., Nov 1999; 274: 33709.
Y. Katayama, J Appl Physiol, Nov 1994; 77: 2086.