All Names
AlternameCDC25HU2, CDC25M2, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2, MPIP2
All SitesHuman: Ser323; Mouse: Ser321; Rat: Ser319
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human CDC25B around the phosphorylation site of serine 323 (S-P-SP-M-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityCDC25B (Phospho-Ser323) antibody detects endogenous levels of CDC25B only when phosphorylated at serine 323.
ReferencesStéphanie Dutertre, J. Cell Sci., May 2004; 117: 2523 - 2531.
Véronique Baldin, J. Biol. Chem., Dec 1997; 272: 32731.
Estelle Schmitt, J. Cell Sci., Oct 2006; 119: 4269 - 4275.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells