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Primary Antibodies
Caspase 9 (Phospho-Thr125) Antibody
Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 92%, Positives = 92%)
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
$225/ 100μg
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Other Names APAF-3; apoptotic protease activating factor 3; apoptotic protease Mch-6; CASP-9; caspase-9; caspase9; EC 3.4.22.62; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; RNCASP9
All Sites Human: Thr125; Mouse: Thr163; Rat: Thr163
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Caspase 9 (Phospho-Thr125) antibody detects endogenous levels of Caspase 9 only when phosphorylated at threonine 125.
ReferencesDuan H., J. Biol. Chem. 271:16720-16724(1996).
Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996).
Hadano S., Mamm. Genome 10:757-760(1999).
Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996).
Hadano S., Mamm. Genome 10:757-760(1999).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Caspase (Phospho-Thr125) antibody.
Western blot analysis of extracts from HeLa 9 cells, treated with TNF (20ng/ml, 5mins) and calyculinA (50ng/ml, 15mins), using Caspase 9 (Phospho-Thr125) antibody.
Conjugated Antibody
This product is also available conjugated. Format: E11-0060A-Conjugate. Price: $485 / Size: 100μl.
E11-0060A-FITC$485 / 100μlE11-0060A-Biotin$485 / 100μlE11-0060A-HRP$485 / 100μlE11-0060A-AP$485 / 100μlE11-0060A-AF350$485 / 100μlE11-0060A-AF405M$485 / 100μlE11-0060A-AF488$485 / 100μlE11-0060A-AF514$485 / 100μlE11-0060A-AF532$485 / 100μlE11-0060A-AF555$485 / 100μlE11-0060A-AF568$485 / 100μlE11-0060A-AF405S$485 / 100μlE11-0060A-AF405L$485 / 100μlE11-0060A-AF546$485 / 100μlE11-0060A-AF594$485 / 100μlE11-0060A-AF610$485 / 100μlE11-0060A-AF635$485 / 100μlE11-0060A-AF647$485 / 100μlE11-0060A-AF680$485 / 100μlE11-0060A-AF700$485 / 100μlE11-0060A-AF750$485 / 100μlE11-0060A-AF790$485 / 100μlE11-0060A-APC$485 / 100μlE11-0060A-PE$485 / 100μlE11-0060A-Cy3$485 / 100μlE11-0060A-Cy5$485 / 100μlE11-0060A-Cy5.5$485 / 100μlE11-0060A-Cy7$485 / 100μlE11-0060A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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