Other Names Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, Caspase-8 precursor, EC 3.4.22.-, FADD-homologous ICE/CED-3- like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, ICE8, MACH, MCH5, MORT1-associated CED-3 homolog
All Sites Human: Ser347; Mouse: Ser349
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Caspase 8 around the phosphorylation site of serine 347 (C-P-SP-L-A).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Caspase 8 (Phospho-Ser347) antibody detects endogenous levels of Caspase 8 only when phosphorylated at serine 347.
ReferencesDaniel R. Beisner, J. Immunol., Sep 2005; 175: 3469 - 3473.
Peter J. Wild, Mol. Cancer Ther., Apr 2005; 4: 516 - 528.
A.V. TIMOFEEVA, Ann. N.Y. Acad. Sci., Dec 2006; 1091: 319 - 335.