All Names
AlternameAGP/EBP, C/EBP beta, C/EBP-related protein 2, CCAAT/enhancer binding protein beta, CEBPB, CRP2, IL-6DBP, Interleukin-6- dependent binding protein, LAP, Liver-enriched transcriptional activator, Nuclear factor NF-IL6, SF-B, SFB, Silencer factor B, TCF5, Transcription factor 5
All SitesHuman: Thr235; Mouse: Thr188; Rat: Thr189
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human C/EBP-β around the phosphorylation site of threonine 235/188 (P-G-TP-P-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityC/EBP-β (phospho-Thr235/188) antibody detects endogenous levels of C/EBP-β only when phosphorylated at threonine 235/188.
ReferencesSongyun Zhu, PNAS, Jan 2002; 99: 207.
Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.
Béatrice Thomas, Eur. J. Biochem., Dec 2000; 267: 6798.
Edoardo Parrella, J. Biol. Chem., Oct 2004; 279: 42026 - 42040.