All Names
AlternameBDNF/NT-3 growth factors receptor precursor, EC 2.7.10.1, GP145-TrkB, GP145-TrkB/GP95-TrkB, NTRK2, Trk-B, TrkB tyrosine kinase, kinase TrkB
All SitesHuman: Tyr516; Mouse: Tyr515; Rat: Tyr515
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Trk B around the phosphorylation site of tyrosine 515 (P-Q-YP-F-G).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityTrk B (phospho-Tyr515) antibody detects endogenous levels of Trk B only when phosphorylated at tyrosine 515.
ReferencesTina Garofalo, J. Biol. Chem., Dec 1998; 273: 35153.
VI Shifrin and BG Neel, J. Biol. Chem., Dec 1993; 268: 25376 - 25384.
Jan Grimm, J. Cell Biol., Jul 2001; 154: 345.
Jose L. Tomsig and Terry T. Turner, J. Androl, May 2006; 27: 348 - 357.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells