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Primary Antibodies
Smad3 (Phospho-Ser425) Antibody
$225/ 100μg/100μl
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
All Names
AlternameJV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3
All SitesHuman: Ser425; Mouse: Ser425; Rat: Ser425
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 425 (C-S-S-V-SP).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivitySmad3 (phospho-Ser425) antibody detects endogenous levels of Smad3 only when phosphorylated at serine 425.
ReferencesSuvi-Katri Leivonen, AACR Meeting Abstracts, Mar 2004; 2004: 530.
Baljit Walia, FASEB J, Sep 2003; 10.1096/fj.02-1211fje.
Evangelia Pardali, J. Biol. Chem., Feb 2000; 275: 3552.
HUGO CEULEMANS, Physiol Rev, Jan 2004; 84: 1.
Baljit Walia, FASEB J, Sep 2003; 10.1096/fj.02-1211fje.
Evangelia Pardali, J. Biol. Chem., Feb 2000; 275: 3552.
HUGO CEULEMANS, Physiol Rev, Jan 2004; 84: 1.
Price225$
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3 (phospho-Ser425) antibody.
Western blot analysis of extracts from NIH/3T3 cells treated with IGF-I (100ng/ml, 10mins), using Smad3 (phospho-Ser425) antibody.
Conjugated Antibody
This product is also available conjugated. Format: E11-0031A-Conjugate. Price: $485 / Size: 100μl.
E11-0031A-FITC$485 / 100μlE11-0031A-Biotin$485 / 100μlE11-0031A-HRP$485 / 100μlE11-0031A-AP$485 / 100μlE11-0031A-AF350$485 / 100μlE11-0031A-AF405M$485 / 100μlE11-0031A-AF488$485 / 100μlE11-0031A-AF514$485 / 100μlE11-0031A-AF532$485 / 100μlE11-0031A-AF555$485 / 100μlE11-0031A-AF568$485 / 100μlE11-0031A-AF405S$485 / 100μlE11-0031A-AF405L$485 / 100μlE11-0031A-AF546$485 / 100μlE11-0031A-AF594$485 / 100μlE11-0031A-AF610$485 / 100μlE11-0031A-AF635$485 / 100μlE11-0031A-AF647$485 / 100μlE11-0031A-AF680$485 / 100μlE11-0031A-AF700$485 / 100μlE11-0031A-AF750$485 / 100μlE11-0031A-AF790$485 / 100μlE11-0031A-APC$485 / 100μlE11-0031A-PE$485 / 100μlE11-0031A-Cy3$485 / 100μlE11-0031A-Cy5$485 / 100μlE11-0031A-Cy5.5$485 / 100μlE11-0031A-Cy7$485 / 100μlE11-0031A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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