Smad2 (Ab-467) Antibody

Name: Smad2 (Ab-467) Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E11-0030B
Price: 225 USD
Availability: InStock

E11-0030BCite ID: EN0016482Datasheet COA MSDS

ReactivityHuman, Mouse, Rat

ApplicationsWB: 1:300~1000 IHC: 1:50~1:100
IF: 1:100~1:500 ELISA: 1:5000

Swiss-Prot No.Q15796

$225/ 100μg/100μl

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (4)Documents

All Names

AlternameJV18-1, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, Smad 2

All SitesHuman: Ser467; Mouse: Ser467; Rat: Ser467

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/StabilityStore at -20℃/1 year

ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of serine 467 (C-S-S-M-SP).

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivitySmad2 (Phospho-Ser467) antibody detects endogenous levels of Smad2 only when phosphorylated at serine 467.

ReferencesKamaraju AK, J Biol Chem 2005 Jan 14; 280(2): 1024-36. Ottesen JJ, Biochemistry 2004 May 18; 43(19): 5698-706. Bourguignon LY, J Biol Chem 2002 Oct 18; 277(42): 39703-12.
Souchelnytskyi S, J Biol Chem 1997 Oct; 272(44): 28107-15.

Price225$

Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

Western Blot analysis of COLO cells using Smad2 Polyclonal Antibody diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,Smad2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 ° C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Hela cell. 1,Smad2 Polyclonal Antibody(red) was diluted at 1:200(4 ℃ overnight). MICU1 Monoclonal Antibody(Mix)(green) was diluted at 1:200(4 ℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

Western blot analysis of Smad2 Polyclonal Antibody, using Hela, HepG2,SW480, HEK293 cell, 4℃ over night, secondary antibody was diluted at 1:10000, 37℃ 1 hour.

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E11-0030B-Conjugate. Price: $485 / Size: 100μl.

E11-0030B-FITC$485 / 100μlE11-0030B-Biotin$485 / 100μlE11-0030B-HRP$485 / 100μlE11-0030B-AP$485 / 100μlE11-0030B-AF350$485 / 100μlE11-0030B-AF405M$485 / 100μlE11-0030B-AF488$485 / 100μlE11-0030B-AF514$485 / 100μlE11-0030B-AF532$485 / 100μlE11-0030B-AF555$485 / 100μlE11-0030B-AF568$485 / 100μlE11-0030B-AF405S$485 / 100μlE11-0030B-AF405L$485 / 100μlE11-0030B-AF546$485 / 100μlE11-0030B-AF594$485 / 100μlE11-0030B-AF610$485 / 100μlE11-0030B-AF635$485 / 100μlE11-0030B-AF647$485 / 100μlE11-0030B-AF680$485 / 100μlE11-0030B-AF700$485 / 100μlE11-0030B-AF750$485 / 100μlE11-0030B-AF790$485 / 100μlE11-0030B-APC$485 / 100μlE11-0030B-PE$485 / 100μlE11-0030B-Cy3$485 / 100μlE11-0030B-Cy5$485 / 100μlE11-0030B-Cy5.5$485 / 100μlE11-0030B-Cy7$485 / 100μlE11-0030B-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.