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Primary Antibodies

Smad2 (Phospho-Ser467) Antibody

E11-0030ACite ID: EN0016481DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1000 IHC: 1:50~1:100
ELISA: 1:5000
Q15796
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameJV18-1, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, Smad 2
All SitesHuman: Ser467; Mouse: Ser467; Rat: Ser467

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of serine 467 (C-S-S-M-SP).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivitySmad2 (Phospho-Ser467) antibody detects endogenous levels of Smad2 only when phosphorylated at serine 467.
ReferencesKamaraju AK, J Biol Chem 2005 Jan 14; 280(2): 1024-36. Ottesen JJ, Biochemistry 2004 May 18; 43(19): 5698-706. Bourguignon LY, J Biol Chem 2002 Oct 18; 277(42): 39703-12.
Souchelnytskyi S, J Biol Chem 1997 Oct; 272(44): 28107-15.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad2 (Phospho- Ser467) antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad2 (Phospho- Ser467) antibody.
Western blot analysis of extracts from HeLa cells treated with PMA (125ng/ml, 15mins), using Smad2 (Phospho- Ser467) antibody.
Western blot analysis of extracts from HeLa cells treated with PMA (125ng/ml, 15mins), using Smad2 (Phospho- Ser467) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0030A-Conjugate. Price: $485 / Size: 100μl.

E11-0030A-FITC$485 / 100μlE11-0030A-Biotin$485 / 100μlE11-0030A-HRP$485 / 100μlE11-0030A-AP$485 / 100μlE11-0030A-AF350$485 / 100μlE11-0030A-AF405M$485 / 100μlE11-0030A-AF488$485 / 100μlE11-0030A-AF514$485 / 100μlE11-0030A-AF532$485 / 100μlE11-0030A-AF555$485 / 100μlE11-0030A-AF568$485 / 100μlE11-0030A-AF405S$485 / 100μlE11-0030A-AF405L$485 / 100μlE11-0030A-AF546$485 / 100μlE11-0030A-AF594$485 / 100μlE11-0030A-AF610$485 / 100μlE11-0030A-AF635$485 / 100μlE11-0030A-AF647$485 / 100μlE11-0030A-AF680$485 / 100μlE11-0030A-AF700$485 / 100μlE11-0030A-AF750$485 / 100μlE11-0030A-AF790$485 / 100μlE11-0030A-APC$485 / 100μlE11-0030A-PE$485 / 100μlE11-0030A-Cy3$485 / 100μlE11-0030A-Cy5$485 / 100μlE11-0030A-Cy5.5$485 / 100μlE11-0030A-Cy7$485 / 100μlE11-0030A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.