Other Names BSP-1, BSP1, Dwarfin-A, Dwf-A, JV4-1, MADH1, MADR1, Mad-related protein 1, Mad1, Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, Mothers-against-DPP-related-1, SMAD 1, Smad 1, Transforming growth factor- beta signaling protein-1
All Sites Human: Ser465; Mouse: Ser465; Rat: Ser468
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Smad1 (Phospho-Ser465) antibody detects endogenous levels of Smad1 only when phosphorylated at serine 465.
ReferencesTino D. Piscione, Am J Physiol Renal Physiol, Jan 2001; 280: 19.
Evangelia Pardali, J. Biol. Chem., Feb 2000; 275: 3552.
Joan Seoane, Carcinogenesis, Nov 2006; 27: 2148 - 2156.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells