World supplier of antibodies, antigens and kits since 2007
Primary Antibodies

Shc (Phospho-Tyr427) Antibody

E11-0025ACite ID: EN0016470DatasheetCOAMSDS
Human
WB: 1:500~1000 IHC: 1:50~100
ELISA: 1:10000
P29353
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameSH2 domain protein C1, SHC, SHC-transforming protein 1, SHCA, Src homology 2 domain-containing-transforming protein C1
All SitesHuman: Tyr427

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Shc around the phosphorylation site of tyrosine 427 (P-S-YP-V-N).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityShc (phospho-Tyr427) antibody detects endogenous levels of Shc only when phosphorylated at serine 24.
ReferencesKerstin Wagner, Carcinogenesis, 2004 25(12):2473-2478.
William A. Ricketts, Mol. Endocrinol., Dec 1999; 13: 1988.
Aimee W. Kao, Endocrinology, Jun 1997; 138: 2474.
Lyn Oligino J. Biol. Chem., Nov 1997; 272: 29046.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human brain tissue using Shc (phospho-Tyr427) antibody.
Immunohistochemical analysis of paraffin-embedded human brain tissue using Shc (phospho-Tyr427) antibody.
Western blot analysis of extracts from Hela cells treated with Calyculin A (50ng/ml, 15mins), using Shc (phospho-Tyr427) antibody.
Western blot analysis of extracts from Hela cells treated with Calyculin A (50ng/ml, 15mins), using Shc (phospho-Tyr427) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0025A-Conjugate. Price: $485 / Size: 100μl.

E11-0025A-FITC$485 / 100μlE11-0025A-Biotin$485 / 100μlE11-0025A-HRP$485 / 100μlE11-0025A-AP$485 / 100μlE11-0025A-AF350$485 / 100μlE11-0025A-AF405M$485 / 100μlE11-0025A-AF488$485 / 100μlE11-0025A-AF514$485 / 100μlE11-0025A-AF532$485 / 100μlE11-0025A-AF555$485 / 100μlE11-0025A-AF568$485 / 100μlE11-0025A-AF405S$485 / 100μlE11-0025A-AF405L$485 / 100μlE11-0025A-AF546$485 / 100μlE11-0025A-AF594$485 / 100μlE11-0025A-AF610$485 / 100μlE11-0025A-AF635$485 / 100μlE11-0025A-AF647$485 / 100μlE11-0025A-AF680$485 / 100μlE11-0025A-AF700$485 / 100μlE11-0025A-AF750$485 / 100μlE11-0025A-AF790$485 / 100μlE11-0025A-APC$485 / 100μlE11-0025A-PE$485 / 100μlE11-0025A-Cy3$485 / 100μlE11-0025A-Cy5$485 / 100μlE11-0025A-Cy5.5$485 / 100μlE11-0025A-Cy7$485 / 100μlE11-0025A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.