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Primary Antibodies

PKC ζ (Phospho-Thr410) Antibody

E11-0023ACite ID: EN0016466DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1000 IHC: 1:50~100
IF: 1:100~1:500 ELISA: 1:20000
Q05513
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameEC 2.7.11.13, KPCZ, PKC-zeta, PKC2, PRKCZ, Protein kinase C zeta type, kinase PKC-zeta, nPKC-zeta
All SitesHuman: Thr410; Mouse: Thr410; Rat: Thr410

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human PKCζ around the phosphorylation site of threonine 410 (T-S-TP-F-C).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityPKCζ (phospho-Thr410) antibody detects endogenous levels of PKCζ only when phosphorylated at threonine 410.
ReferencesTodd E. Fox, J. Biol. Chem., Feb 2007; 10.1074/jbc.M700082200.
Katherine A. Fitzgerald, J. Immunol., Feb 2000; 164: 2053.
Koichi Asanuma, J. Immunol., Mar 2004; 172: 3922.
Ashish Bhattacharjee, J. Immunol., Sep 2006; 177: 3771 - 3781.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCζ (phospho- Thr410) antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCζ (phospho- Thr410) antibody.
Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using PKCζ (phospho-Thr410) antibody.
Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using PKCζ (phospho-Thr410) antibody.
Immunofluorescence analysis of HeLa cells, using PKCζ (phospho-Thr410) antibody.
Immunofluorescence analysis of HeLa cells, using PKCζ (phospho-Thr410) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0023A-Conjugate. Price: $485 / Size: 100μl.

E11-0023A-FITC$485 / 100μlE11-0023A-Biotin$485 / 100μlE11-0023A-HRP$485 / 100μlE11-0023A-AP$485 / 100μlE11-0023A-AF350$485 / 100μlE11-0023A-AF405M$485 / 100μlE11-0023A-AF488$485 / 100μlE11-0023A-AF514$485 / 100μlE11-0023A-AF532$485 / 100μlE11-0023A-AF555$485 / 100μlE11-0023A-AF568$485 / 100μlE11-0023A-AF405S$485 / 100μlE11-0023A-AF405L$485 / 100μlE11-0023A-AF546$485 / 100μlE11-0023A-AF594$485 / 100μlE11-0023A-AF610$485 / 100μlE11-0023A-AF635$485 / 100μlE11-0023A-AF647$485 / 100μlE11-0023A-AF680$485 / 100μlE11-0023A-AF700$485 / 100μlE11-0023A-AF750$485 / 100μlE11-0023A-AF790$485 / 100μlE11-0023A-APC$485 / 100μlE11-0023A-PE$485 / 100μlE11-0023A-Cy3$485 / 100μlE11-0023A-Cy5$485 / 100μlE11-0023A-Cy5.5$485 / 100μlE11-0023A-Cy7$485 / 100μlE11-0023A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.