Other Names DNA-binding factor KBF1, EBP-1, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1, NFkB-p50, Nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit
All Sites Human: Ser927; Mouse: Ser930
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p105/p50 around the phosphorylation site of serine 927 (C-D-SP-G-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity NF-κB p105/p50 (phospho-Ser927) antibody detects endogenous levels of NF-κB p105/p50 only when phosphorylated at serine 927.
ReferencesTheresa Joseph, Am J Physiol Lung Cell Mol Physiol, Mar 2005; 288: L471 - L479.
Sabine Kirchhoff, Eur. J. Biochem., Apr 1999; 261: 546.
Gerald Thiel, Eur. J. Biochem., Jul 2004; 271: 2855 - 2862.