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Primary Antibodies

NF-κB p105/p50 (phospho-Ser927) Antibody

E11-0021ACite ID: EN0016462DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000
P19838
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names DNA-binding factor KBF1, EBP-1, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1, NFkB-p50, Nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit
All Sites Human: Ser927; Mouse: Ser930
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p105/p50 around the phosphorylation site of serine 927 (C-D-SP-G-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity NF-κB p105/p50 (phospho-Ser927) antibody detects endogenous levels of NF-κB p105/p50 only when phosphorylated at serine 927.
ReferencesTheresa Joseph, Am J Physiol Lung Cell Mol Physiol, Mar 2005; 288: L471 - L479.
Sabine Kirchhoff, Eur. J. Biochem., Apr 1999; 261: 546.
Gerald Thiel, Eur. J. Biochem., Jul 2004; 271: 2855 - 2862.
Price225$
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p105/p50 (phospho-Ser927) antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p105/p50 (phospho-Ser927) antibody.
Western blot analysis of extracts from Hela cells treated with LPS (100ng/ml, 30min), using NF-κB p105/p50 (phospho-Ser927) antibody .
Western blot analysis of extracts from Hela cells treated with LPS (100ng/ml, 30min), using NF-κB p105/p50 (phospho-Ser927) antibody .
Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins), using NF-κB p105/p50 (phospho-Ser927) antibody.
Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins), using NF-κB p105/p50 (phospho-Ser927) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0021A-Conjugate. Price: $485 / Size: 100μl.

E11-0021A-FITC$485 / 100μlE11-0021A-Biotin$485 / 100μlE11-0021A-HRP$485 / 100μlE11-0021A-AP$485 / 100μlE11-0021A-AF350$485 / 100μlE11-0021A-AF405M$485 / 100μlE11-0021A-AF488$485 / 100μlE11-0021A-AF514$485 / 100μlE11-0021A-AF532$485 / 100μlE11-0021A-AF555$485 / 100μlE11-0021A-AF568$485 / 100μlE11-0021A-AF405S$485 / 100μlE11-0021A-AF405L$485 / 100μlE11-0021A-AF546$485 / 100μlE11-0021A-AF594$485 / 100μlE11-0021A-AF610$485 / 100μlE11-0021A-AF635$485 / 100μlE11-0021A-AF647$485 / 100μlE11-0021A-AF680$485 / 100μlE11-0021A-AF700$485 / 100μlE11-0021A-AF750$485 / 100μlE11-0021A-AF790$485 / 100μlE11-0021A-APC$485 / 100μlE11-0021A-PE$485 / 100μlE11-0021A-Cy3$485 / 100μlE11-0021A-Cy5$485 / 100μlE11-0021A-Cy5.5$485 / 100μlE11-0021A-Cy7$485 / 100μlE11-0021A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.