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Primary Antibodies

Keratin 8 (Phospho-Ser73) Antibody

E11-0017ACite ID: EN0016454DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1000 IHC: 1:50~100
ELISA: 1:40000
P05787
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameCK 8, CK8, CYK8, Cytokeratin endo A, K2C8, K8, KRT2-8, KRT8, Keratin, type II cytoskeletal 8, cytokeratin 8
All SitesHuman: Ser73; Mouse: Ser79

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Keratin 8 around the phosphorylation site of serine 73 (L-L-SP-P-L).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityKeratin 8 (phospho-Ser73) antibody detects endogenous levels of Keratin 8 only when phosphorylated at serine 73.
ReferencesNam-On Ku J. Biol. Chem., Mar 2002; 277: 10775 - 10782.
Karen M. Ridge J. Biol. Chem., Aug 2005; 280: 30400 - 30405.
Nam-On Ku J. Biol. Chem., Mar 1997; 272: 7556.
Gary K. Scott Mol. Pharmacol., Sep 2005; 68: 606 - 615.
Price225$
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 (phospho-Ser73) antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 (phospho-Ser73) antibody.
Western blot analysis of extracts from 293 cells treated with Etoposide (25uM, 60mins), using Keratin 8 (phospho-Ser73) antibody.
Western blot analysis of extracts from 293 cells treated with Etoposide (25uM, 60mins), using Keratin 8 (phospho-Ser73) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0017A-Conjugate. Price: $485 / Size: 100μl.

E11-0017A-FITC$485 / 100μlE11-0017A-Biotin$485 / 100μlE11-0017A-HRP$485 / 100μlE11-0017A-AP$485 / 100μlE11-0017A-AF350$485 / 100μlE11-0017A-AF405M$485 / 100μlE11-0017A-AF488$485 / 100μlE11-0017A-AF514$485 / 100μlE11-0017A-AF532$485 / 100μlE11-0017A-AF555$485 / 100μlE11-0017A-AF568$485 / 100μlE11-0017A-AF405S$485 / 100μlE11-0017A-AF405L$485 / 100μlE11-0017A-AF546$485 / 100μlE11-0017A-AF594$485 / 100μlE11-0017A-AF610$485 / 100μlE11-0017A-AF635$485 / 100μlE11-0017A-AF647$485 / 100μlE11-0017A-AF680$485 / 100μlE11-0017A-AF700$485 / 100μlE11-0017A-AF750$485 / 100μlE11-0017A-AF790$485 / 100μlE11-0017A-APC$485 / 100μlE11-0017A-PE$485 / 100μlE11-0017A-Cy3$485 / 100μlE11-0017A-Cy5$485 / 100μlE11-0017A-Cy5.5$485 / 100μlE11-0017A-Cy7$485 / 100μlE11-0017A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.