All Names
AlternameCK 8, CK8, CYK8, Cytokeratin endo A, K2C8, K8, KRT2-8, KRT8, Keratin, type II cytoskeletal 8, cytokeratin 8
All SitesHuman: Ser73; Mouse: Ser79
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Keratin 8 around the phosphorylation site of serine 73 (L-L-SP-P-L).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityKeratin 8 (phospho-Ser73) antibody detects endogenous levels of Keratin 8 only when phosphorylated at serine 73.
ReferencesNam-On Ku J. Biol. Chem., Mar 2002; 277: 10775 - 10782.
Karen M. Ridge J. Biol. Chem., Aug 2005; 280: 30400 - 30405.
Nam-On Ku J. Biol. Chem., Mar 1997; 272: 7556.
Gary K. Scott Mol. Pharmacol., Sep 2005; 68: 606 - 615.