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Primary Antibodies

Keratin 18 (Phospho-Ser33) Antibody

E11-0016ACite ID: EN0016452DatasheetCOAMSDS
Human
WB: 1:500~1000 IHC: 1:50~1:100
IF: 1:100~1:500 ELISA: 1:5000
P05783
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameCK 18, CK18, CYK18, Cytokeratin endo B, K18, K1C18, KERD, KRT1-18, KRT18, Keratin, Keratin D, Keratin, type I cytoskeletal 18, cytokeratin 18, type I cytoskeletal 18
All SitesHuman: Ser33

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Keratin 18 around the phosphorylation site of serine 33 (A-A-SP-V-Y).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityKeratin 18 (phospho-Ser33) antibody detects endogenous levels of Keratin 18 only when phosphorylated at serine 33.
ReferencesJ Liao, J. Cell Biol., Dec 1995; 131: 1291.
CF Chou and MB Omary, J. Biol. Chem., Feb 1993; 268: 4465 - 4472.
NO Ku and MB Omary, J. Cell Biol., Oct 1994; 127: 161.
Nam-On Ku J. Cell Biol., May 2000; 149: 547.
Price225$
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin (phospho-Ser33) antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin (phospho-Ser33) antibody.
Western blot analysis of extracts from HT29 cells, 18 using Keratin 18 (phospho-Ser33) antibody.
Western blot analysis of extracts from HT29 cells, 18 using Keratin 18 (phospho-Ser33) antibody.
Immunofluorescence analysis of HeLa cells, using Keratin 18 (phospho-Ser33) antibody.
Immunofluorescence analysis of HeLa cells, using Keratin 18 (phospho-Ser33) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0016A-Conjugate. Price: $485 / Size: 100μl.

E11-0016A-FITC$485 / 100μlE11-0016A-Biotin$485 / 100μlE11-0016A-HRP$485 / 100μlE11-0016A-AP$485 / 100μlE11-0016A-AF350$485 / 100μlE11-0016A-AF405M$485 / 100μlE11-0016A-AF488$485 / 100μlE11-0016A-AF514$485 / 100μlE11-0016A-AF532$485 / 100μlE11-0016A-AF555$485 / 100μlE11-0016A-AF568$485 / 100μlE11-0016A-AF405S$485 / 100μlE11-0016A-AF405L$485 / 100μlE11-0016A-AF546$485 / 100μlE11-0016A-AF594$485 / 100μlE11-0016A-AF610$485 / 100μlE11-0016A-AF635$485 / 100μlE11-0016A-AF647$485 / 100μlE11-0016A-AF680$485 / 100μlE11-0016A-AF700$485 / 100μlE11-0016A-AF750$485 / 100μlE11-0016A-AF790$485 / 100μlE11-0016A-APC$485 / 100μlE11-0016A-PE$485 / 100μlE11-0016A-Cy3$485 / 100μlE11-0016A-Cy5$485 / 100μlE11-0016A-Cy5.5$485 / 100μlE11-0016A-Cy7$485 / 100μlE11-0016A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.