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Primary Antibodies

IκB-ε (Phospho-Ser22) Antibody

E11-0015ACite ID: EN0016450DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
IF: 1:100~1:500 ELISA: 1:40000
O00221
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameI-kappa-B-epsilon, IKBE, IkappaBepsilon, NF-kappa-BIE, NF-kappaB inhibitor epsilon, NFKBIE
All SitesHuman: Ser22; Mouse: Ser22; Rat: Ser22

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human IκB-ε around the phosphorylation site of serine 22 (I-E-SP-L-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityIκB-ε (phospho-Ser22) antibody detects endogenous levels of IκB-ε only when phosphorylated at serine 22.
ReferencesDavid C. Hay Mol, Cell. Biol., May 2001; 21: 3482 - 3490.
Fumitaka Fujita Mol. Cell. Biol., Nov 2003; 23: 7780 - 7793.
Dan Tapalaga J. Histochem. Cytochem., Dec 2002; 50: 1599.
Price225$
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using (phospho-Ser22) antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using (phospho-Ser22) antibody.
Western blot analysis of extracts from Jurkat cells, IκB-ε treated with TNF-a (20ng/ml, 30mins), using IκB-ε (phospho-Ser22) antibody.
Western blot analysis of extracts from Jurkat cells, IκB-ε treated with TNF-a (20ng/ml, 30mins), using IκB-ε (phospho-Ser22) antibody.
Immunofluorescence analysis of HeLa cells, using IκB-ε (phospho-Ser22) antibody.
Immunofluorescence analysis of HeLa cells, using IκB-ε (phospho-Ser22) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0015A-Conjugate. Price: $485 / Size: 100μl.

E11-0015A-FITC$485 / 100μlE11-0015A-Biotin$485 / 100μlE11-0015A-HRP$485 / 100μlE11-0015A-AP$485 / 100μlE11-0015A-AF350$485 / 100μlE11-0015A-AF405M$485 / 100μlE11-0015A-AF488$485 / 100μlE11-0015A-AF514$485 / 100μlE11-0015A-AF532$485 / 100μlE11-0015A-AF555$485 / 100μlE11-0015A-AF568$485 / 100μlE11-0015A-AF405S$485 / 100μlE11-0015A-AF405L$485 / 100μlE11-0015A-AF546$485 / 100μlE11-0015A-AF594$485 / 100μlE11-0015A-AF610$485 / 100μlE11-0015A-AF635$485 / 100μlE11-0015A-AF647$485 / 100μlE11-0015A-AF680$485 / 100μlE11-0015A-AF700$485 / 100μlE11-0015A-AF750$485 / 100μlE11-0015A-AF790$485 / 100μlE11-0015A-APC$485 / 100μlE11-0015A-PE$485 / 100μlE11-0015A-Cy3$485 / 100μlE11-0015A-Cy5$485 / 100μlE11-0015A-Cy5.5$485 / 100μlE11-0015A-Cy7$485 / 100μlE11-0015A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.