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Primary Antibodies

GSK3α/β(Phospho-Tyr279/216) Antibody

E11-0012ACite ID: EN0016445DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
P49840
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameEC 2.7.11.26, Factor A, GSK-3 alpha, GSK3-alpha, Glycogen synthase kinase-3 alpha, kinase GSK3-alpha, GSK-3 beta, GSK3B, Glycogen synthase kinase-3 beta, Protein kinase GSK-3-beta, kinase GSK3-beta
All SitesHuman: Tyr279/216; Mouse: Tyr279/216; Rat: Tyr279/216

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human GSK3α/β around the phosphorylation site of tyrosine 279/216 (V-S-YP-I-C).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityGSK3α/β (phospho-Tyr279/216) antibody detects endogenous levels of GSK3α/β only when phosphorylated at tyrosine 279/216.
ReferencesMinsub Shim, J. Biol. Chem., May 2003; 278: 19674 - 19681.
Junichi Gotoh Carcinogenesis, Mar 2003; 24: 435 - 442.
Katrina MacAulay, J. Biol. Chem., Mar 2005; 280: 9509 - 9518.
Victor H. Bustos, PNAS, Dec 2006; 103: 19725 - 19730.
Price225$
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using GSK3α/β (phospho-Tyr279/216) antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using GSK3α/β (phospho-Tyr279/216) antibody.
Western blot analysis of extracts from HeLa cells, using GSK3α/β (phospho-Tyr279/216) antibody.
Western blot analysis of extracts from HeLa cells, using GSK3α/β (phospho-Tyr279/216) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0012A-Conjugate. Price: $485 / Size: 100μl.

E11-0012A-FITC$485 / 100μlE11-0012A-Biotin$485 / 100μlE11-0012A-HRP$485 / 100μlE11-0012A-AP$485 / 100μlE11-0012A-AF350$485 / 100μlE11-0012A-AF405M$485 / 100μlE11-0012A-AF488$485 / 100μlE11-0012A-AF514$485 / 100μlE11-0012A-AF532$485 / 100μlE11-0012A-AF555$485 / 100μlE11-0012A-AF568$485 / 100μlE11-0012A-AF405S$485 / 100μlE11-0012A-AF405L$485 / 100μlE11-0012A-AF546$485 / 100μlE11-0012A-AF594$485 / 100μlE11-0012A-AF610$485 / 100μlE11-0012A-AF635$485 / 100μlE11-0012A-AF647$485 / 100μlE11-0012A-AF680$485 / 100μlE11-0012A-AF700$485 / 100μlE11-0012A-AF750$485 / 100μlE11-0012A-AF790$485 / 100μlE11-0012A-APC$485 / 100μlE11-0012A-PE$485 / 100μlE11-0012A-Cy3$485 / 100μlE11-0012A-Cy5$485 / 100μlE11-0012A-Cy5.5$485 / 100μlE11-0012A-Cy7$485 / 100μlE11-0012A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.