All Names
AlternameEC 2.7.11.26, Factor A, GSK-3 alpha, GSK3-alpha, Glycogen synthase kinase-3 alpha, kinase GSK3-alpha, GSK-3 beta, GSK3B, Glycogen synthase kinase-3 beta, Protein kinase GSK-3-beta, kinase GSK3-beta
All SitesHuman: Tyr279/216; Mouse: Tyr279/216; Rat: Tyr279/216
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human GSK3α/β around the phosphorylation site of tyrosine 279/216 (V-S-YP-I-C).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityGSK3α/β (phospho-Tyr279/216) antibody detects endogenous levels of GSK3α/β only when phosphorylated at tyrosine 279/216.
ReferencesMinsub Shim, J. Biol. Chem., May 2003; 278: 19674 - 19681.
Junichi Gotoh Carcinogenesis, Mar 2003; 24: 435 - 442.
Katrina MacAulay, J. Biol. Chem., Mar 2005; 280: 9509 - 9518.
Victor H. Bustos, PNAS, Dec 2006; 103: 19725 - 19730.