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Primary Antibodies

EGFR (Phospho-Thr678) Antibody

E11-0008ACite ID: EN0016435DatasheetCOAMSDS
Human, Mouse
WB: 1:300~1:1000 IF: 1:100~1:500
ELISA: 1:40000
P00533
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameEC 2.7.10.1, ERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR
All SitesHuman: Thr678; Mouse: Thr680

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of threonine 678 (K-R-TP-L-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityEGFR (phospho-Thr678) antibody detects endogenous levels of EGFR only when phosphorylated at threonine 678.
ReferencesRoland Reinehr, FASEB J, Feb 2003; 10: 1096.
Natsumi Yamamoto, J. Cell Sci., Nov 2006; 119: 4623 - 4633.
James G. Christensen Clin, Cancer Res., Dec 2001; 7: 4230.
Hideki Katayama, AACR Meeting Abstracts, Mar 2004; 2004: 1074.
Ali Tadlaoui Hbibi, AACR Meeting Abstracts, Apr 2006; 2006: 1177
Komal Raina, AACR Meeting Abstracts, Apr 2006; 2006: 752 - 753.
Price225$
Western blot analysis of extracts from cos7 cells treated with EGF (200ng/ml, 30mins), using EGFR (phospho-Thr678) antibody.
Western blot analysis of extracts from cos7 cells treated with EGF (200ng/ml, 30mins), using EGFR (phospho-Thr678) antibody.
Immunofluorescence analysis of HuvEc cells, using EGFR (phospho-Thr678) antibody.
Immunofluorescence analysis of HuvEc cells, using EGFR (phospho-Thr678) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0008A-Conjugate. Price: $485 / Size: 100μl.

E11-0008A-FITC$485 / 100μlE11-0008A-Biotin$485 / 100μlE11-0008A-HRP$485 / 100μlE11-0008A-AP$485 / 100μlE11-0008A-AF350$485 / 100μlE11-0008A-AF405M$485 / 100μlE11-0008A-AF488$485 / 100μlE11-0008A-AF514$485 / 100μlE11-0008A-AF532$485 / 100μlE11-0008A-AF555$485 / 100μlE11-0008A-AF568$485 / 100μlE11-0008A-AF405S$485 / 100μlE11-0008A-AF405L$485 / 100μlE11-0008A-AF546$485 / 100μlE11-0008A-AF594$485 / 100μlE11-0008A-AF610$485 / 100μlE11-0008A-AF635$485 / 100μlE11-0008A-AF647$485 / 100μlE11-0008A-AF680$485 / 100μlE11-0008A-AF700$485 / 100μlE11-0008A-AF750$485 / 100μlE11-0008A-AF790$485 / 100μlE11-0008A-APC$485 / 100μlE11-0008A-PE$485 / 100μlE11-0008A-Cy3$485 / 100μlE11-0008A-Cy5$485 / 100μlE11-0008A-Cy5.5$485 / 100μlE11-0008A-Cy7$485 / 100μlE11-0008A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.