All Names
AlternameEC 2.7.10.1, ERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR
All SitesHuman: Thr678; Mouse: Thr680
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of threonine 678 (K-R-TP-L-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityEGFR (phospho-Thr678) antibody detects endogenous levels of EGFR only when phosphorylated at threonine 678.
ReferencesRoland Reinehr, FASEB J, Feb 2003; 10: 1096.
Natsumi Yamamoto, J. Cell Sci., Nov 2006; 119: 4623 - 4633.
James G. Christensen Clin, Cancer Res., Dec 2001; 7: 4230.
Hideki Katayama, AACR Meeting Abstracts, Mar 2004; 2004: 1074.
Ali Tadlaoui Hbibi, AACR Meeting Abstracts, Apr 2006; 2006: 1177
Komal Raina, AACR Meeting Abstracts, Apr 2006; 2006: 752 - 753.