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Primary Antibodies

DARPP-32 (Phospho-Thr75) Antibody

E11-0007ACite ID: EN0016433DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1000 IHC: 1:50~1:100
ELISA: 1:40000
Q9UD71
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameDopamine- and cAMP-regulated neuronal phosphoprotein, IPPD, Neuronal phosphoprotein DARPP-32, PPP1R1B
All SitesHuman:Thr75; Mouse: Thr75; Rat: Thr75

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityDARPP-32 (phospho-Thr75) antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 75.
ReferencesJA Girault, J. Biol. Chem., Dec 1989; 264: 21748 - 21759.
GL Snyder, J. Neurosci., Aug 1992; 12: 3071.
Akinori Nishi, J. Neurosci., Nov 1997; 17: 8147.
Artur Mayerhofer, J. Clin. Endocrinol. Metab., Dec 2000; 85: 4750 - 4757.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human brain tissue using DARPP-32 (phospho-Thr75) antibody.
Immunohistochemical analysis of paraffin-embedded human brain tissue using DARPP-32 (phospho-Thr75) antibody.
Western blot analysis of extracts from COS7 cells treated with Forskolin (40nM, 30mins), using DARPP-32 (phospho-Thr75) antibody.
Western blot analysis of extracts from COS7 cells treated with Forskolin (40nM, 30mins), using DARPP-32 (phospho-Thr75) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0007A-Conjugate. Price: $485 / Size: 100μl.

E11-0007A-FITC$485 / 100μlE11-0007A-Biotin$485 / 100μlE11-0007A-HRP$485 / 100μlE11-0007A-AP$485 / 100μlE11-0007A-AF350$485 / 100μlE11-0007A-AF405M$485 / 100μlE11-0007A-AF488$485 / 100μlE11-0007A-AF514$485 / 100μlE11-0007A-AF532$485 / 100μlE11-0007A-AF555$485 / 100μlE11-0007A-AF568$485 / 100μlE11-0007A-AF405S$485 / 100μlE11-0007A-AF405L$485 / 100μlE11-0007A-AF546$485 / 100μlE11-0007A-AF594$485 / 100μlE11-0007A-AF610$485 / 100μlE11-0007A-AF635$485 / 100μlE11-0007A-AF647$485 / 100μlE11-0007A-AF680$485 / 100μlE11-0007A-AF700$485 / 100μlE11-0007A-AF750$485 / 100μlE11-0007A-AF790$485 / 100μlE11-0007A-APC$485 / 100μlE11-0007A-PE$485 / 100μlE11-0007A-Cy3$485 / 100μlE11-0007A-Cy5$485 / 100μlE11-0007A-Cy5.5$485 / 100μlE11-0007A-Cy7$485 / 100μlE11-0007A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.