All Names
Altername5'-AMP-activated protein kinase, catalytic alpha-1 chain, AAPK1, AMPK alpha-1 chain, AMPK-alpha1, EC 2.7.11.1, HMG-CoA redustase kinase, PRKAA1, acetyl-CoA carboxylase kinase, hormone-sensitive lipase kinase, kinase AMPK-alpha1
All SitesHuman: Thr172; Mouse: Thr172; Rat: Thr172
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human AMPKα around the phosphorylation site of threonine172 (L-R-TP-S-C).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityAMPKα (Phospho-Thr172) antibody detects endogenous levels of AMPKα only when phosphorylated at threonine172 in both chain 1 and 2.
ReferencesSiwang Yu AACR, Meeting Abstracts, Apr 2006; 2006: 538.
Laura BarréAm, J Physiol Endocrinol Metab, Mar 2007; 292: E802 - E811.
Hideyuki Sakoda, Am J Physiol Endocrinol Metab, Sep 2005; 289: E474 - E481.
Lucie Tosca Endocrinology, Oct 2005; 146: 4500 - 4513.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells