14-3-3 ζ (Phospho-Ser58) Antibody

Name: 14-3-3 ζ (Phospho-Ser58) Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E11-0001A

E11-0001ACite ID: EN0016420Datasheet COA MSDS

ReactivityHuman, Mouse, Rat

ApplicationsWB: 1:500~1000 IHC: 1:50~100IF: 1:100~1:500 ELISA: 1:40000

Swiss-Prot No.P63104

Price on request

Quantity

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (4)Documents

All Names

Altername14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ

All SitesHuman: Ser58; Mouse: Ser58; Rat: Ser58

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/StabilityStore at -20℃/1 year

ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 ζ around the phosphorylation site of serine 58 (R-S-SP-W-R).

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity14-3-3 ζ (phospho-Ser58) antibody detects endogenous levels of 14-3-3 ζ only when phosphorylated at serine 58.

ReferencesYing H. Shen Mol. Biol. Cell, Nov 2003; 14: 4721.
David W. Powell, Mol. Cell. Biol., Aug 2003; 23: 5376 - 5387.
Mackintosh. C, (2004) Biochem. J. 381, 329 - 42.
Dougherty, M.K. and Morrison, D.K. (2004) J. Cell Sci. 117, 1875 - 84.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 ζ (phospho-Ser58) antibody.

Western blot analysis of e extracts from NIH/3T3 cells treated with UV (30mins), using 14-3-3 ζ (phospho-Ser58) antibody.

with Western blot analysis using 14-3-3 ζ ζ (Phospho-Ser58) Antibody. Line 1: Blocking peptide; Line 2: The extracts from A549 cells ; Line 3: The extracts from 3T3 cells.

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E11-0001A-Conjugate. Price: $485 / Size: 100μl.

E11-0001A-FITC$485 / 100μlE11-0001A-Biotin$485 / 100μlE11-0001A-HRP$485 / 100μlE11-0001A-AP$485 / 100μlE11-0001A-AF350$485 / 100μlE11-0001A-AF405M$485 / 100μlE11-0001A-AF488$485 / 100μlE11-0001A-AF514$485 / 100μlE11-0001A-AF532$485 / 100μlE11-0001A-AF555$485 / 100μlE11-0001A-AF568$485 / 100μlE11-0001A-AF405S$485 / 100μlE11-0001A-AF405L$485 / 100μlE11-0001A-AF546$485 / 100μlE11-0001A-AF594$485 / 100μlE11-0001A-AF610$485 / 100μlE11-0001A-AF635$485 / 100μlE11-0001A-AF647$485 / 100μlE11-0001A-AF680$485 / 100μlE11-0001A-AF700$485 / 100μlE11-0001A-AF750$485 / 100μlE11-0001A-AF790$485 / 100μlE11-0001A-APC$485 / 100μlE11-0001A-PE$485 / 100μlE11-0001A-Cy3$485 / 100μlE11-0001A-Cy5$485 / 100μlE11-0001A-Cy5.5$485 / 100μlE11-0001A-Cy7$485 / 100μlE11-0001A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.