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Primary Antibodies
MAP3K5 Antibody
Human
WB, IHC, IF, FC, ELISA. Not yet tested in other applications.
$225/ 100μg/100μl
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
BackgroundMitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Product Information
Clone Number2E4
SpeciesMouse IgG1
Entrez Gene4217
Recommended DilutionsWB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, IF: 1/200-1/1000, FC: 1/200 - 1/400,
ELISA: Propose dilution 1/10000.
ELISA: Propose dilution 1/10000.
Molecular Weight55kDa
All Names
AlternameASK1; MEKK5; MAPKKK5
ImmunogenPurified recombinant fragment of human MAP3K5 expressed in E. Coli.
Storage/StabilityStore at 4℃, for long term storage, store at -20℃.
Form Or Antibody
Form of AntibodyAscitic fluid containing 0.03% sodium azide.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Figure 1: Western blot analysis using MAP3K5 mAb against recombinant protein.
analysis of paraffin-embedded breast cancer tissues (right) using MAP3K5 mouse mAb with DAB staining.
analysis of paraffin-embedded breast cancer tissues (right) using MAP3K5 mouse mAb with DAB staining.
Conjugated Antibody
This product is also available conjugated. Format: E10-30225-Conjugate. Price: $485 / Size: 100μl.
E10-30225-FITC$485 / 100μlE10-30225-Biotin$485 / 100μlE10-30225-HRP$485 / 100μlE10-30225-AP$485 / 100μlE10-30225-AF350$485 / 100μlE10-30225-AF405M$485 / 100μlE10-30225-AF488$485 / 100μlE10-30225-AF514$485 / 100μlE10-30225-AF532$485 / 100μlE10-30225-AF555$485 / 100μlE10-30225-AF568$485 / 100μlE10-30225-AF405S$485 / 100μlE10-30225-AF405L$485 / 100μlE10-30225-AF546$485 / 100μlE10-30225-AF594$485 / 100μlE10-30225-AF610$485 / 100μlE10-30225-AF635$485 / 100μlE10-30225-AF647$485 / 100μlE10-30225-AF680$485 / 100μlE10-30225-AF700$485 / 100μlE10-30225-AF750$485 / 100μlE10-30225-AF790$485 / 100μlE10-30225-APC$485 / 100μlE10-30225-PE$485 / 100μlE10-30225-Cy3$485 / 100μlE10-30225-Cy5$485 / 100μlE10-30225-Cy5.5$485 / 100μlE10-30225-Cy7$485 / 100μlE10-30225-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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