World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
HIF1A Antibody
Human; Mouse; Monkey
WB, IHC, IF, ELISA. Not yet tested in other applications.
$225/ 100μg/100μl
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
BackgroundHypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. (provided by RefSeq) Tissue specificity: Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
Product Information
Clone Number1A3
SpeciesMouse IgG1
Entrez Gene3091
Recommended DilutionsWB: 1/500 - 1/2000, IHC: 1/200-1/1000, IF: 1/200-1/1000,
ELISA: Propose dilution 1/10000.
ELISA: Propose dilution 1/10000.
Molecular Weight120kDa
All Names
AlternameHIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF1A
ImmunogenPurified recombinant fragment of human HIF1A expressed in E. Coli.
Storage/StabilityStore at 4℃, for long term storage, store at -20℃.
Form Or Antibody
Form of AntibodyAscitic fluid containing 0.03% sodium azide.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Figure 1: Western blot analysis using HIF1A mouse mAb against Cos7 (1), Hela (2), Jurkat (3), RAJI (4) and NIH/3T3 (5) cell lysate.
Immunohistochemical analysis of paraffin-embedded liver cancer (left) and lung cancer tissues (right) using HIF1A mouse mAb with staining.
2: Immunohistochemical analysis of paraffin-embedded liver (left) and lung cancer tissues (right) using HIF1A mouse mAb staining.
Conjugated Antibody
This product is also available conjugated. Format: E10-30195-Conjugate. Price: $485 / Size: 100μl.
E10-30195-FITC$485 / 100μlE10-30195-Biotin$485 / 100μlE10-30195-HRP$485 / 100μlE10-30195-AP$485 / 100μlE10-30195-AF350$485 / 100μlE10-30195-AF405M$485 / 100μlE10-30195-AF488$485 / 100μlE10-30195-AF514$485 / 100μlE10-30195-AF532$485 / 100μlE10-30195-AF555$485 / 100μlE10-30195-AF568$485 / 100μlE10-30195-AF405S$485 / 100μlE10-30195-AF405L$485 / 100μlE10-30195-AF546$485 / 100μlE10-30195-AF594$485 / 100μlE10-30195-AF610$485 / 100μlE10-30195-AF635$485 / 100μlE10-30195-AF647$485 / 100μlE10-30195-AF680$485 / 100μlE10-30195-AF700$485 / 100μlE10-30195-AF750$485 / 100μlE10-30195-AF790$485 / 100μlE10-30195-APC$485 / 100μlE10-30195-PE$485 / 100μlE10-30195-Cy3$485 / 100μlE10-30195-Cy5$485 / 100μlE10-30195-Cy5.5$485 / 100μlE10-30195-Cy7$485 / 100μlE10-30195-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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