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Primary Antibodies
EIF4E Antibody
Human
WB, IHC, IF, FC, ELISA. Not yet tested in other applications.
$225/ 100μg/100μl
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
BackgroundeIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
Product Information
Clone Number5D11
SpeciesMouse IgG1
Entrez Gene1977
Recommended DilutionsWB: 1/500 - 1/2000, IHC: 1/200-1/1000, IF: 1/200-1/1000, FC: 1/200-1/400,
ELISA: Propose dilution 1/10000.
ELISA: Propose dilution 1/10000.
Molecular Weight25kDa
All Names
AlternameCBP; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E
ImmunogenPurified recombinant fragment of human EIF4E expressed in E. Coli.
Storage/StabilityStore at 4℃, for long term storage, store at -20℃.
Form Or Antibody
Form of AntibodyAscitic fluid containing 0.03% sodium azide.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Figure 1: Western blot analysis using EIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) using EIF4E mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded liver cancer submaxillary tumor (right) using EIF4E mouse mAb with DAB
Conjugated Antibody
This product is also available conjugated. Format: E10-30189-Conjugate. Price: $485 / Size: 100μl.
E10-30189-FITC$485 / 100μlE10-30189-Biotin$485 / 100μlE10-30189-HRP$485 / 100μlE10-30189-AP$485 / 100μlE10-30189-AF350$485 / 100μlE10-30189-AF405M$485 / 100μlE10-30189-AF488$485 / 100μlE10-30189-AF514$485 / 100μlE10-30189-AF532$485 / 100μlE10-30189-AF555$485 / 100μlE10-30189-AF568$485 / 100μlE10-30189-AF405S$485 / 100μlE10-30189-AF405L$485 / 100μlE10-30189-AF546$485 / 100μlE10-30189-AF594$485 / 100μlE10-30189-AF610$485 / 100μlE10-30189-AF635$485 / 100μlE10-30189-AF647$485 / 100μlE10-30189-AF680$485 / 100μlE10-30189-AF700$485 / 100μlE10-30189-AF750$485 / 100μlE10-30189-AF790$485 / 100μlE10-30189-APC$485 / 100μlE10-30189-PE$485 / 100μlE10-30189-Cy3$485 / 100μlE10-30189-Cy5$485 / 100μlE10-30189-Cy5.5$485 / 100μlE10-30189-Cy7$485 / 100μlE10-30189-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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