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Primary Antibodies

PRKAA1 Antibody

E10-30172Cite ID: EN0013850DatasheetCOAMSDS
Human; Monkey; Mouse; Rat
WB, IHC, IF, FC, ELISA. Not yet tested in other applications.
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

BackgroundThe protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Information

Clone Number2B7
SpeciesMouse IgG1
Entrez Gene5562
Recommended DilutionsWB: 1/500 - 1/2000, IHC: 1/200-1/1000, IF: 1/200-1/1000, FC: 1/200-1/400,
ELISA: Propose dilution 1/10000.
Molecular Weight64kDa

All Names

AlternameAMPK; AMPKa1; MGC33776; MGC57364; PRKAA1
ImmunogenPurified recombinant fragment of human PRKAA1 expressed in E. Coli.
Storage/StabilityStore at 4℃, for long term storage, store at -20℃.

Form Or Antibody

Form of AntibodyAscitic fluid containing 0.03% sodium azide.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Figure 1: Western blot analysis using PRKAA1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
Figure 1: Western blot analysis using PRKAA1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.
(left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.
(left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.

Conjugated Antibody

This product is also available conjugated. Format: E10-30172-Conjugate. Price: $485 / Size: 100μl.

E10-30172-FITC$485 / 100μlE10-30172-Biotin$485 / 100μlE10-30172-HRP$485 / 100μlE10-30172-AP$485 / 100μlE10-30172-AF350$485 / 100μlE10-30172-AF405M$485 / 100μlE10-30172-AF488$485 / 100μlE10-30172-AF514$485 / 100μlE10-30172-AF532$485 / 100μlE10-30172-AF555$485 / 100μlE10-30172-AF568$485 / 100μlE10-30172-AF405S$485 / 100μlE10-30172-AF405L$485 / 100μlE10-30172-AF546$485 / 100μlE10-30172-AF594$485 / 100μlE10-30172-AF610$485 / 100μlE10-30172-AF635$485 / 100μlE10-30172-AF647$485 / 100μlE10-30172-AF680$485 / 100μlE10-30172-AF700$485 / 100μlE10-30172-AF750$485 / 100μlE10-30172-AF790$485 / 100μlE10-30172-APC$485 / 100μlE10-30172-PE$485 / 100μlE10-30172-Cy3$485 / 100μlE10-30172-Cy5$485 / 100μlE10-30172-Cy5.5$485 / 100μlE10-30172-Cy7$485 / 100μlE10-30172-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.