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Primary Antibodies
SMAD5 Antibody
Human; Rat
WB, IHC, IF, FC, ELISA. Not yet tested in other applications.
$225/ 100μg/100μl
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
BackgroundTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo; lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.
Product Information
Clone Number3H9
SpeciesMouse IgG1
Entrez Gene4090
Recommended DilutionsWB: 1/500 - 1/2000, IHC: 1/200-1/1000, IF: 1/200-1/1000, FC: 1/200-1/400,
ELISA: Propose dilution 1/10000.
ELISA: Propose dilution 1/10000.
Molecular Weight52kDa
All Names
AlternameDwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5
ImmunogenPurified recombinant fragment of human SMAD5 expressed in E. Coli.
Storage/StabilityStore at 4℃, for long term storage, store at -20℃.
Form Or Antibody
Form of AntibodyAscitic fluid containing 0.03% sodium azide.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Figure 1: Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
Immunohistochemical analysis of paraffin-embedded brain tissues lung cancer tissues (right) using SMAD5 mouse mAb with DAB
Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have with Alexa Fluor-555 phalloidin.
Conjugated Antibody
This product is also available conjugated. Format: E10-30150-Conjugate. Price: $485 / Size: 100μl.
E10-30150-FITC$485 / 100μlE10-30150-Biotin$485 / 100μlE10-30150-HRP$485 / 100μlE10-30150-AP$485 / 100μlE10-30150-AF350$485 / 100μlE10-30150-AF405M$485 / 100μlE10-30150-AF488$485 / 100μlE10-30150-AF514$485 / 100μlE10-30150-AF532$485 / 100μlE10-30150-AF555$485 / 100μlE10-30150-AF568$485 / 100μlE10-30150-AF405S$485 / 100μlE10-30150-AF405L$485 / 100μlE10-30150-AF546$485 / 100μlE10-30150-AF594$485 / 100μlE10-30150-AF610$485 / 100μlE10-30150-AF635$485 / 100μlE10-30150-AF647$485 / 100μlE10-30150-AF680$485 / 100μlE10-30150-AF700$485 / 100μlE10-30150-AF750$485 / 100μlE10-30150-AF790$485 / 100μlE10-30150-APC$485 / 100μlE10-30150-PE$485 / 100μlE10-30150-Cy3$485 / 100μlE10-30150-Cy5$485 / 100μlE10-30150-Cy5.5$485 / 100μlE10-30150-Cy7$485 / 100μlE10-30150-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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