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Primary Antibodies

HK1 Antibody

E10-30089Cite ID: EN0013763DatasheetCOAMSDS
Human; Mouse; Rat
WB, IHC, IF, FC, ELISA. Not yet tested in other applications.
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

BackgroundThe hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines.

Product Information

Clone Number3A10
SpeciesMouse IgG1
Entrez Gene3098
Recommended DilutionsWB: 1/500 - 1/2000, IHC: 1/200-1/1000, IF: 1/200 - 1/1000, FC: 1/200-1/400,
ELISA: Propose dilution 1/10000.
Molecular Weight120kDa

All Names

AlternameHKI; HXK1; HK1-ta; HK1-tb; HK1-tc; HK1
ImmunogenPurified recombinant fragment of human HK1 expressed in E. Coli.
Storage/StabilityStore at 4℃, for long term storage, store at -20℃.

Form Or Antibody

Form of AntibodyAscitic fluid containing 0.03% sodium azide.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Figure 1: Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) Figure 3: cell lysate. using HK1 For Research
Figure 1: Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) Figure 3: cell lysate. using HK1 For Research
Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have with Alexa Fluor-555 phalloidin.
Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis of paraffin-embedded kidney tissues mouse mAb with DAB staining. Use Only
Immunohistochemical analysis of paraffin-embedded kidney tissues mouse mAb with DAB staining. Use Only

Conjugated Antibody

This product is also available conjugated. Format: E10-30089-Conjugate. Price: $485 / Size: 100μl.

E10-30089-FITC$485 / 100μlE10-30089-Biotin$485 / 100μlE10-30089-HRP$485 / 100μlE10-30089-AP$485 / 100μlE10-30089-AF350$485 / 100μlE10-30089-AF405M$485 / 100μlE10-30089-AF488$485 / 100μlE10-30089-AF514$485 / 100μlE10-30089-AF532$485 / 100μlE10-30089-AF555$485 / 100μlE10-30089-AF568$485 / 100μlE10-30089-AF405S$485 / 100μlE10-30089-AF405L$485 / 100μlE10-30089-AF546$485 / 100μlE10-30089-AF594$485 / 100μlE10-30089-AF610$485 / 100μlE10-30089-AF635$485 / 100μlE10-30089-AF647$485 / 100μlE10-30089-AF680$485 / 100μlE10-30089-AF700$485 / 100μlE10-30089-AF750$485 / 100μlE10-30089-AF790$485 / 100μlE10-30089-APC$485 / 100μlE10-30089-PE$485 / 100μlE10-30089-Cy3$485 / 100μlE10-30089-Cy5$485 / 100μlE10-30089-Cy5.5$485 / 100μlE10-30089-Cy7$485 / 100μlE10-30089-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.