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Primary Antibodies
CDC2 Antibody
Human
WB, IF, FC, ELISA. Not yet tested in other applications.
$225/ 100μg/100μl
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
BackgroundThe cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues.
Product Information
Clone Number8C5A7F10
SpeciesMouse IgG1
Entrez Gene983
Recommended DilutionsWB: 1/500 - 1/2000, IF: 1/200-1/1000, FC: 1/200-1/400, ELISA: Propose dilution 1/10000.
Molecular Weight34kDa
All Names
AlternameCDC2; CDC28A; P34CDC2; MGC111195; DKFZp686L20222; CDK1
ImmunogenPurified recombinant fragment of CDC2 expressed in E. Coli.
Storage/StabilityStore at 4℃, for long term storage, store at -20℃.
Form Or Antibody
Form of AntibodyAscitic fluid containing 0.03% sodium azide.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Figure 1: Western blot analysis using CDC2 mouse mAb against Jurkat cell lysate. Figure 3: Flow and negative control
Immunofluorescence analysis of Hela cells using CDC2 mouse mAb filaments have been labeled with Alexa Fluor-555 phalloidin.
cytometric analysis of PC-2 cells using CDC2 mouse mAb (green) (purple). Use Only
Conjugated Antibody
This product is also available conjugated. Format: E10-30040-Conjugate. Price: $485 / Size: 100μl.
E10-30040-FITC$485 / 100μlE10-30040-Biotin$485 / 100μlE10-30040-HRP$485 / 100μlE10-30040-AP$485 / 100μlE10-30040-AF350$485 / 100μlE10-30040-AF405M$485 / 100μlE10-30040-AF488$485 / 100μlE10-30040-AF514$485 / 100μlE10-30040-AF532$485 / 100μlE10-30040-AF555$485 / 100μlE10-30040-AF568$485 / 100μlE10-30040-AF405S$485 / 100μlE10-30040-AF405L$485 / 100μlE10-30040-AF546$485 / 100μlE10-30040-AF594$485 / 100μlE10-30040-AF610$485 / 100μlE10-30040-AF635$485 / 100μlE10-30040-AF647$485 / 100μlE10-30040-AF680$485 / 100μlE10-30040-AF700$485 / 100μlE10-30040-AF750$485 / 100μlE10-30040-AF790$485 / 100μlE10-30040-APC$485 / 100μlE10-30040-PE$485 / 100μlE10-30040-Cy3$485 / 100μlE10-30040-Cy5$485 / 100μlE10-30040-Cy5.5$485 / 100μlE10-30040-Cy7$485 / 100μlE10-30040-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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