World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
HSPA5 Antibody
Human
WB, IHC, ELISA. Not yet tested in other applications.
$225/ 100μg/100μl
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
BackgroundWhen Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.The HSP70 proteins are ubiquitous molecular chaparones that are found in all organisms and tissue types. Like other members of the HSP70 family, BiP is a peptide-binding ATPase that is able to differentiate native proteins from unfolded polypeptides. BiP does not bind to fully folded and assembled proteins, except in the presence of other co-chaparones. BiP is involved in a number of key mechanisms and pathways including polypeptide translocation across the endoplasmic reticulum, folding, assembly, transport of secreted or membrane proteins, and the regulation of calcium homeostasis. Although BiP is relatively abundant, marked increases in BiP occur where there is an accumulation of unfolded polypeptides.
Product Information
Clone Number4E3
SpeciesMouse IgG1
Entrez Gene3309
Recommended DilutionsWB: 1/500 - 1/2000, IHC: 1/200-1/1000, ELISA: Propose dilution 1/10000.
Molecular Weight78kDa
All Names
AlternameBIP; MIF2; GRP78; FLJ26106; HSPA5
ImmunogenPurified recombinant fragment of human HSPA5 expressed in E. Coli.
Storage/StabilityStore at 4℃, for long term storage, store at -20℃.
Form Or Antibody
Form of AntibodyAscitic fluid containing 0.03% sodium azide.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Figure 1: Western blot analysis using HSPA5 mouse mAb against NIH/3T3 (1), Hela (2) and Jurkat (3) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin- embedded human Thyroid tissues using HSPA5 mouse mAb.
Conjugated Antibody
This product is also available conjugated. Format: E10-30028-Conjugate. Price: $485 / Size: 100μl.
E10-30028-FITC$485 / 100μlE10-30028-Biotin$485 / 100μlE10-30028-HRP$485 / 100μlE10-30028-AP$485 / 100μlE10-30028-AF350$485 / 100μlE10-30028-AF405M$485 / 100μlE10-30028-AF488$485 / 100μlE10-30028-AF514$485 / 100μlE10-30028-AF532$485 / 100μlE10-30028-AF555$485 / 100μlE10-30028-AF568$485 / 100μlE10-30028-AF405S$485 / 100μlE10-30028-AF405L$485 / 100μlE10-30028-AF546$485 / 100μlE10-30028-AF594$485 / 100μlE10-30028-AF610$485 / 100μlE10-30028-AF635$485 / 100μlE10-30028-AF647$485 / 100μlE10-30028-AF680$485 / 100μlE10-30028-AF700$485 / 100μlE10-30028-AF750$485 / 100μlE10-30028-AF790$485 / 100μlE10-30028-APC$485 / 100μlE10-30028-PE$485 / 100μlE10-30028-Cy3$485 / 100μlE10-30028-Cy5$485 / 100μlE10-30028-Cy5.5$485 / 100μlE10-30028-Cy7$485 / 100μlE10-30028-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
Categories by research area
Categories by product type
- Antibodies
- Primary Antibodies
- Tag antibodies
- Internal control antibodies
- Pathological (Immunohistochemistry, IHC) antibodies
- Cell Signal Transduction Research Antibodies
- Secondary Antibody
- Antibody Sampler Kits
- ELISA Kits
- Recombinant Proteins
- Fluorescence Reagents
- Signal Inhibitors and Activators
- Transfection Reagent
- Cell biology kits
- Overexpression Cell Lines
- Cell Lysates
- Purification Resins
- 2019-nCoV & Monkeypox