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Primary Antibodies

Pirh2 Antibody

E10-30005Cite ID: EN0013677DatasheetCOAMSDS
Human; Rat
WB, IHC, IF, FC, ELISA. Not yet tested in other applications.
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

BackgroundPirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.

Product Information

Clone Number1H10
SpeciesMouse IgG1
Entrez Gene25898
Recommended DilutionsWB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, IF: 1/200-1/1000, FC: 1/200-1/400,
ELISA: Propose dilution 1/10000.
Molecular Weight30kDa; 60kDa (homodimer)

All Names

AlternameARNIP; CHIMP; RNF199; RCHY1
ImmunogenPurified recombinant fragment of human Pirh2 expressed in E. Coli.
Storage/StabilityStore at 4℃, for long term storage, store at -20℃.

Form Or Antibody

Form of AntibodyAscitic fluid containing 0.03% sodium azide.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate. Figure 3: Flow and negative
Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate. Figure 3: Flow and negative
Immunohistochemical analysis of paraffin-embedded human Tonsil anti-Pirh2 mouse mAb.
Immunohistochemical analysis of paraffin-embedded human Tonsil anti-Pirh2 mouse mAb.
cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) control (red).
cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) control (red).

Conjugated Antibody

This product is also available conjugated. Format: E10-30005-Conjugate. Price: $485 / Size: 100μl.

E10-30005-FITC$485 / 100μlE10-30005-Biotin$485 / 100μlE10-30005-HRP$485 / 100μlE10-30005-AP$485 / 100μlE10-30005-AF350$485 / 100μlE10-30005-AF405M$485 / 100μlE10-30005-AF488$485 / 100μlE10-30005-AF514$485 / 100μlE10-30005-AF532$485 / 100μlE10-30005-AF555$485 / 100μlE10-30005-AF568$485 / 100μlE10-30005-AF405S$485 / 100μlE10-30005-AF405L$485 / 100μlE10-30005-AF546$485 / 100μlE10-30005-AF594$485 / 100μlE10-30005-AF610$485 / 100μlE10-30005-AF635$485 / 100μlE10-30005-AF647$485 / 100μlE10-30005-AF680$485 / 100μlE10-30005-AF700$485 / 100μlE10-30005-AF750$485 / 100μlE10-30005-AF790$485 / 100μlE10-30005-APC$485 / 100μlE10-30005-PE$485 / 100μlE10-30005-Cy3$485 / 100μlE10-30005-Cy5$485 / 100μlE10-30005-Cy5.5$485 / 100μlE10-30005-Cy7$485 / 100μlE10-30005-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.