Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced by immunizing rabbit with synthesized peptide containing the Flag epitope (D-Y-K-D-D-D-D-K).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Specificty Sensitivity
Specificity/SensitivityFlag-Tag polyclonal antibody detects Flag-tagged proteins overexpressed in cells. The antibody recognizes the Flag-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells.
ReferencesTerpe K. (2003) Appl Microbiol Biotechnol; 60(5):523-533.
Einhauer A, et al. (2001) J Biochem Biophys Methods; 49(1-3):455-465.
Brizzard, B.L. et al. (1994) Biotechniques; 16:730–735.