World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
His-Tag Polyclonal Antibody
All
WB: 1:500~1:1000
$150/ 100μg/100μl
Specification
QuantityTotal: $150
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced by immunizing rabbit with synthesized peptide containing the His epitope (H-H-H-H-H-H).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Specificty Sensitivity
Specificity/SensitivityHis-Tag polyclonal antibody detects His-tagged proteins overexpressed in cells. The antibody recognizes the His-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells.
ReferencesTerpe K. et al. (2003) Appl Microbiol Biotechnol; 60(5):523-533.
Xu L. et al. (2006) Cell Mol Immunol; 3(2):139-143.
Els Conrath, K., et al. ( 2001) J. Biol. Chem. 276: 7346-7350.
Xu L. et al. (2006) Cell Mol Immunol; 3(2):139-143.
Els Conrath, K., et al. ( 2001) J. Biol. Chem. 276: 7346-7350.
Price150$
Western blot analysis of extract from control 293 cells (Lane 1) or transfected with His-tagged S6 (Lane 2) and transfected with His-tagged CREB (Lane 3) using His-Tag polyclonal antibody (E0T502).
Conjugated Antibody
This product is also available conjugated. Format: E0T502-Conjugate. Price: $485 / Size: 100μl.
E0T502-FITC$485 / 100μlE0T502-Biotin$485 / 100μlE0T502-HRP$485 / 100μlE0T502-AP$485 / 100μlE0T502-AF350$485 / 100μlE0T502-AF405M$485 / 100μlE0T502-AF488$485 / 100μlE0T502-AF514$485 / 100μlE0T502-AF532$485 / 100μlE0T502-AF555$485 / 100μlE0T502-AF568$485 / 100μlE0T502-AF405S$485 / 100μlE0T502-AF405L$485 / 100μlE0T502-AF546$485 / 100μlE0T502-AF594$485 / 100μlE0T502-AF610$485 / 100μlE0T502-AF635$485 / 100μlE0T502-AF647$485 / 100μlE0T502-AF680$485 / 100μlE0T502-AF700$485 / 100μlE0T502-AF750$485 / 100μlE0T502-AF790$485 / 100μlE0T502-APC$485 / 100μlE0T502-PE$485 / 100μlE0T502-Cy3$485 / 100μlE0T502-Cy5$485 / 100μlE0T502-Cy5.5$485 / 100μlE0T502-Cy7$485 / 100μlE0T502-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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