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Primary Antibodies

4E-BP1 (Ab-45) Antibody

E021216-1Cite ID: EN0002575DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
Q13541
$100/ 50μg/50μl
Specification
Quantity
Total: $100

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificty Sensitivity

Specificity/Sensitivity4E-BP1 (Ab-45) antibody detects endogenous levels of total 4E-BP1 protein. This antibody may cross-react with 4E-BP2 and 4E-BP3.
ReferencesGingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.
Price100$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-45) antibody (E021216).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-45) antibody (E021216).
Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with EGF (200ng/ml, 5min), using 4E-BP1 (Ab-45) antibody (E021216, Lane1 and 2) and 4E-BP1 (phospho-Thr45) antibody (E011223, Lane 3 and 4).
Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with EGF (200ng/ml, 5min), using 4E-BP1 (Ab-45) antibody (E021216, Lane1 and 2) and 4E-BP1 (phospho-Thr45) antibody (E011223, Lane 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E021216-1-Conjugate. Price: $485 / Size: 100μl.

E021216-1-FITC$485 / 100μlE021216-1-Biotin$485 / 100μlE021216-1-HRP$485 / 100μlE021216-1-AP$485 / 100μlE021216-1-AF350$485 / 100μlE021216-1-AF405M$485 / 100μlE021216-1-AF488$485 / 100μlE021216-1-AF514$485 / 100μlE021216-1-AF532$485 / 100μlE021216-1-AF555$485 / 100μlE021216-1-AF568$485 / 100μlE021216-1-AF405S$485 / 100μlE021216-1-AF405L$485 / 100μlE021216-1-AF546$485 / 100μlE021216-1-AF594$485 / 100μlE021216-1-AF610$485 / 100μlE021216-1-AF635$485 / 100μlE021216-1-AF647$485 / 100μlE021216-1-AF680$485 / 100μlE021216-1-AF700$485 / 100μlE021216-1-AF750$485 / 100μlE021216-1-AF790$485 / 100μlE021216-1-APC$485 / 100μlE021216-1-PE$485 / 100μlE021216-1-Cy3$485 / 100μlE021216-1-Cy5$485 / 100μlE021216-1-Cy5.5$485 / 100μlE021216-1-Cy7$485 / 100μlE021216-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.