Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 612 (P-M-SP-P-G).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityIRS-1 (Phospho-Ser612) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 612.
ReferencesAndreozzi F, et al. (2004) Endocrinology 145, 2845-57.
Esposito DL, Li Y, Cama A, Quon MJ (2001) Endocrinology 142, 2833-40.
Rikova K, et al. (2007) Cell 131, 1190-203.
Sun, X.J. et al. (1991) Nature 352, 73-77.
Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672.
Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells