Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human IKKa/b around the phosphorylation site of serine 180/181 (C-T-SP-F-V ).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityIKKa/b (Phospho-Ser180/181) antibody detects endogenous levels of IKKa/b only when phosphorylated at serine 180/181.
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Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells