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Primary Antibodies

HDAC1 (Phospho-Ser421/423) Antibody

E012085-1Cite ID: EN0001117DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
Q13547
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HDAC1 around the phosphorylation site of serine 421/423 (E-F-SP-D- SP –E-E).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityHDAC1 (Phospho-Ser421/423) antibody detects endogenous levels of HDAC1 only when phosphorylated at serine 421/423.
ReferencesDephoure N, et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
Han G, et al. (2008) Proteomics 8, 1346-61.
Molina H, et al. (2007) Proc Natl Acad Sci U S A 104, 2199-204.
Olsen JV, et al. (2006) Cell 127, 635-48.
Pflum MK, Tong JK, et al. (2001) J Biol Chem 276, 47733-41.
Marmorstein, R. et al. (2001) Cell. Mol. Life Sci. 58, 693-703.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC1 (Phospho-Ser421/423) antibody (E012085).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC1 (Phospho-Ser421/423) antibody (E012085).
Western blot analysis of extracts from HELA cells. Left:Using HDAC1 (Phospho-Ser421/423) antibody (E012085) . Right: Using the same antibody preincubated with synthesized peptide.
Western blot analysis of extracts from HELA cells. Left:Using HDAC1 (Phospho-Ser421/423) antibody (E012085) . Right: Using the same antibody preincubated with synthesized peptide.

Conjugated Antibody

This product is also available conjugated. Format: E012085-1-Conjugate. Price: $485 / Size: 100μl.

E012085-1-FITC$485 / 100μlE012085-1-Biotin$485 / 100μlE012085-1-HRP$485 / 100μlE012085-1-AP$485 / 100μlE012085-1-AF350$485 / 100μlE012085-1-AF405M$485 / 100μlE012085-1-AF488$485 / 100μlE012085-1-AF514$485 / 100μlE012085-1-AF532$485 / 100μlE012085-1-AF555$485 / 100μlE012085-1-AF568$485 / 100μlE012085-1-AF405S$485 / 100μlE012085-1-AF405L$485 / 100μlE012085-1-AF546$485 / 100μlE012085-1-AF594$485 / 100μlE012085-1-AF610$485 / 100μlE012085-1-AF635$485 / 100μlE012085-1-AF647$485 / 100μlE012085-1-AF680$485 / 100μlE012085-1-AF700$485 / 100μlE012085-1-AF750$485 / 100μlE012085-1-AF790$485 / 100μlE012085-1-APC$485 / 100μlE012085-1-PE$485 / 100μlE012085-1-Cy3$485 / 100μlE012085-1-Cy5$485 / 100μlE012085-1-Cy5.5$485 / 100μlE012085-1-Cy7$485 / 100μlE012085-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.