Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HDAC1 around the phosphorylation site of serine 421/423 (E-F-SP-D- SP –E-E).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityHDAC1 (Phospho-Ser421/423) antibody detects endogenous levels of HDAC1 only when phosphorylated at serine 421/423.
ReferencesDephoure N, et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
Han G, et al. (2008) Proteomics 8, 1346-61.
Molina H, et al. (2007) Proc Natl Acad Sci U S A 104, 2199-204.
Olsen JV, et al. (2006) Cell 127, 635-48.
Pflum MK, Tong JK, et al. (2001) J Biol Chem 276, 47733-41.
Marmorstein, R. et al. (2001) Cell. Mol. Life Sci. 58, 693-703.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells