Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Gab1 around the phosphorylation site of tyrosine 659 (V-D-YP-V-V).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityGab1 (Phospho-Tyr659) antibody detects endogenous levels of Gab1 only when phosphorylated at tyrosine 659.
ReferencesHoffmann KM, Tapia JA, Jensen RT (2006) Cell Signal 18, 942-54.
Montagner A, et al. (2005) J Biol Chem 280, 5350-60.
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Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
Weidner, K.M. et al. (1996) Nature 384, 173-176.
Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells