Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human FGFR1 around the phosphorylation site of RMPVAPYWTSPEK tyrosine 154 (A-P-YP-W-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityFGFR1 (Phospho-Tyr154) antibody detects endogenous levels of FGFR1 only when phosphorylated at tyrosine 154.
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Hinsby AM, Olsen JV, Bennett KL, Mann M (2003) Mol Cell Proteomics 2, 29-36.
Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.
Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8.
Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells